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ABN1446 Anti-GPRIN1

Anti-GPRIN1 Antibody, Cat. No. ABN1446, is a highly specific rabbit polyclonal antibody that targets GPRIN1 and has been tested in Immunohistochemistry and Western Blotting.

Research Category: Neuroscience Gene Symbol: GPRIN1, KIAA1893 UniProt Number: Q7Z2K8
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ABN1446
50 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1446
DescriptionAnti-GPRIN1
Alternate Names
  • G protein-regulated inducer of neurite outgrowth 1
  • GRIN1
Background InformationG protein-regulated inducer of neurite outgrowth 1 (UniProt Q7Z2K8; also known as GRIN1) is encoded by the GPRIN1 (also known as KIAA1893) gene (Gene ID 114787) in human. Originally identified as a binding protein for guanosine 5'-3-O-(thio)triphosphate-bound G(z)alphaz, G protein-regulated inducer of neurite outgrowth 1 (GPRIN1) is a signaling molecule that functions as a downstream target of G(o)alpha. When co-expressed with constitutively active form of G(o)alpha, GPRIN1 is demonstrated to stimulate neurite extensions in Neuro2a cells. Gprin1 expression in mice is limited to the nervous system at all developmental stages, occurring within the entire embryonic mantle zones and becoming restricted to sets of nuclei at postnatal to adult stages. Gprin1 immunoreactivity is found colocalized with G(o)alpha at neuronal dendrites and axons, while being undetected in glial cells. Studies conducted in rat reveals that 7 nicotinic receptor ( 7) significantly reduces axon growth in hippocampal neurons by coupling to Gprin1, which targets it to the growth cone. RNAi-mediated Gprin1 knockdown abolishes the localization and calcium signaling of 7 at the growth cone. The 7/Gprin1 interaction appears intimately linked to a G(o)alpha, growth-associated protein 43, and CDC42 cytoskeletal regulatory pathway within the developing axon.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GPRIN1 Antibody, Cat. No. ABN1446, is a highly specific rabbit polyclonal antibody that targets GPRIN1 and has been tested in Immunohistochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GPRIN1 in human prostate cancer, cerebral cortex and pancreas tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence of human GPRIN1.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody targets an internal sequence present in both spliced isoforms of human GPRIN1 protein reported by UniProt (Q7Z2K8).
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GPRIN1
  • KIAA1893
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~105 kDa observed. 102.4 kDa (isoform 1) and 80.95 kDa (isoform 2) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:20,000 dilution of this antibody detected GPRIN1 in 10 µg of HT-29 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABN1446 04054839056291

Documentation