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MABF2350-25UG Anti-GSDMD Antibody, clone 8C2

Anti-GSDMD, clone 8C2, Cat. No. MABF2350, is a mouse monoclonal antibody that detects Gasdermin-D and is tested for use in Immunocytochemistry and Western Blotting.

MABF2350-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2350-25UG
DescriptionAnti-GSDMD Antibody, clone 8C2
Alternate Names
  • Gasdermin-D
Background InformationGasdermin-D (UniProt: P57764; also known as Gasdermin domain-containing protein 1) is encoded by the GSDMD (also known as DFNA5L, GSDMDC1, FKSG10) gene (Gene ID: 79792) in human. Gasdermin-D is a member of the gasdermin family of proteins that promotes inflammatory cell death (pyroptosis) in response to microbial infection and danger signals. Its expression is observed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach. Gasdermin-2 consists of a 31 kDa N-terminal and a 22 kDa C-terminal domains that are separated by a linker region. Under normal conditions, the full-length Gasdermin-D is inactive as the linker loop between the N-terminal and C-terminal domains stabilizes the overall conformation of the full-length protein and allows the C-terminal domain to fold back on and auto-inhibit the N-terminal Gasdermin-D (Gasdermin-N). Upon interdomain cleavage by the action of caspase-1 and 4 at Asp275, the autoinhibition is relieved and Gasdermin-N cytotoxicity is triggered. Gasdermin-N is reported to form membrane pore that lead to cytokine release and pyroptosis. It also displays bactericidal activity. Gasdermin-N released from pyroptotic cells into the extracellular milieu can bind to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells. (Ref.: Liu, X., et al. (2016). Nature. 535(7610); 153-158; Hu, JJ., et al. (2020). Nat. Immunol. 21(7):736-745).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-GSDMD, clone 8C2, Cat. No. MABF2350, is a mouse monoclonal antibody that detects Gasdermin-D and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected recombinant Gasdermin-D (Courtesy of Judy Lieberman, M.D., Ph.D. and Zhibin Zhang, Ph.D., Boston Children's Hospital/Harvard Medical School, Massachusetts, USA).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length recombinant human Gasdermin-D.
EpitopeUnknown
Clone8C2
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 8C2 is a mouse monoclonal antibody that detects human Gasdermin-D.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GSDMD
  • DFNA5L
  • GSDMDC1
  • FKSG10
Purification MethodProtein G purified
UniProt Number
Molecular Weight52.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HEK293T cells .

Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Gasdermin-D in HEK293T cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF2350-25UG 04065265324311