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MAC111 Anti-GST•Tag™ fusion protein Antibody

This Anti-GST•Tag fusion protein Antibody is validated for use in Western Blotting, ICC, Immunoprecipitation, ELISA for the detection of GST•Tag fusion protein.

Anti-GST•Tag™ fusion protein Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epitope Tags & General Use Research Sub-Category: Epitope Tags UniProt Number: P08515
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MAC111
50 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, ICC, IP, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAC111
DescriptionAnti-GST•Tag™ fusion protein Antibody
Alternate Names
  • Glutathione S-transferase class-mu 26 kDa isozyme
  • GST 26
  • Sj26 antigen
  • SjGST
Background InformationGST (glutathione-S-transferase) represents a major group of detoxification enzymes, which are known to exist as multiple cytosolic and membrane-bound isoenzymes, with distinct catalytic and noncatalytic binding properties. Whereas the cytosolic enzymes are encoded by at least five distantly related gene families, the membrane-bound enzymes are encoded by single genes. GST is regulated and induced by a diverse range of xenobiotics. Many, but not all, inducers of GST, act through either the antioxidant-responsive element (ARE), the GST P enhancer 1(GPE), the xenobiotic-responsive element (XRE), or the glucocorticoid-responsive element (GRE). Barbiturates may transcriptionally activate GST through a Barbie box element. Many of the compounds that induce GST are also GST substrates, or are metabolized by cytochrome P-450 monooxygenases into compounds that can serve as GST substrates. Because the expression levels of GST play a critical role in determining the sensitivity of cells to a broad range of toxic chemicals, GST is suggested to contribute to resistance against carcinogens, antitumor drugs, environmental pollutants, and products of oxidative stress.
References
Product Information
FormatPurified
Control
  • Posi-Tag Epitope Tag Control
PresentationPurified mouse monoclonal IgG1κ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-GST•Tag fusion protein Antibody is validated for use in Western Blotting, ICC, Immunoprecipitation, ELISA for the detection of GST•Tag fusion protein.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
Application NotesImmunocytochemistry Analysis: A representative lot detected GST•Tag fusion protein in transfected COS-1 cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated GST•Tag fusion protein sequence in IP.

ELISA Analysis: A representative lot detected GST•Tag protein sequence in ELISA.
Biological Information
ImmunogenGluththione-S-transferase (GST) domain from Schistosoma Japonicum
Clone8-326.6.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~45 kDa observed. The molecular weight of the GST•Tag fusion protein is not ~45 kDa. The Posi-Tag Epitope Tag Control, which contains the fusion protein, is observed at ~45 kDa in Western Blot.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Posi-Tag Epitope Tag Control.

Western Blot Analysis: This antibody detected GST•Tag fusion protein in 5 µL of Posi-Tag Epitope Tag Control.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAC111 04053252550966