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MABF1969 Anti-Glutathione Peroxidase 4 Antibody, clone Mab63-1

Anti-Glutathione Peroxidase 4, clone Mab63-1, Cat. No. MABF1969, is a mouse monoclonal antibody that detects cytosolic glutathione peroxidase 4 and has been tested for use in Dot Blot, Electron Microscopy, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: GPX4 UniProt Number: P36969-2
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MABF1969
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IP, EM, IH(P), WB, DBMAffinity PurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1969
DescriptionAnti-Glutathione Peroxidase 4 Antibody, clone Mab63-1
Alternate Names
  • Phospholipid hydroperoxide glutathione peroxidase cytosolic
  • cGPx4
  • PHGPx
  • Glutathione peroxidase 4
  • GPx-4
  • GSHPx-4
Background InformationPhospholipid hydroperoxide glutathione peroxidase, cytosolic (UniProt: P36969-2; also known as PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4) is encoded by the GPX4 gene (Gene ID: 2879) in human. Three functionally distinct isozymes of GPx4 have been described and are known as mGPx4 (mitochondrial), cGPx4 (cytosolic), and nGPx4 (nuclear). They originate from a single GPX4 gene.
The cytosolic isoform is ubiquitously expressed at moderate levels in most mammalian cells. On the other hand, mitochondrial and nuclear isoforms are overexpressed in spermatoid cells. Clone Mab63-1 specifically recognizes cGPx4 in human and its ortholog in mouse. However, it does not recognize rat GPx4. GPx4 is a considered to be a moonlighting protein that has been implicated in anti-oxidative defense, gene expression regulation, and programmed cell death, and as a structural protein in spermatogenesis. Homozygous GPx4-deficient mice are reported to die utero before E7.5. Similarly, homozygous mice expressing catalytically inactive GPx4 variants (Sec46Ala/Ser mutant) do not survive beyond the 7th day of embryogenesis. (Ref.: Borchert, A., et al (2010). Immunol. Lett. 133 (2); 85-93).
References
Product Information
FormatAffinity Purified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Glutathione Peroxidase 4, clone Mab63-1, Cat. No. MABF1969, is a mouse monoclonal antibody that detects cytosolic glutathione peroxidase 4 and has been tested for use in Dot Blot, Electron Microscopy, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Electron Microscopy
  • Immunohistochemistry (Paraffin)
  • Western Blotting
  • Dot Blot
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Glutathione Peroxidase 4 in human testis and mouse testis tissues.

Immunoprecipitation Analysis: A representative lot detected Glutathione Peroxidase 4 in Immunoprecipitation applications (Borchert, A., et. al. (2010). Immunol Lett. 133(2):85-93).

Immunohistochemistry Analysis: A representative lot detected Glutathione Peroxidase 4 in Immunohistochemistry applications (Borchert, A., et. al. (2010). Immunol Lett. 133(2):85-93; Brutsch, S.H., et. al. (2015). Antioxid Redox Signal. 22(4):281-93).

Western Blotting Analysis: A representative lot detected Glutathione Peroxidase 4 in Western Blotting applications (Brutsch, S.H., et. al. (2015). Antioxid Redox Signal. 22(4):281-93; Borchert, A., et. al. (2010). Immunol Lett. 133(2):85-93).

Dot Blot Analysis: A representative lot detected Glutathione Peroxidase 4 in Dot Blot applications (Borchert, A., et. al. (2010). Immunol Lett. 133(2):85-93).

Electron Microscopy Analysis: A representative lot detected Glutathione Peroxidase 4 in Electron Microscopy applications (Brutsch, S.H., et. al. (2016). J Biol Chem. 291(45):23578-23588).
Biological Information
ImmunogenHis-tagged purified recombinant human cytosolic glutathione peroxidase 4.
CloneMab63-1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone Mab63-1 specifically detects cytosolic Glutathione Peroxidase 4 in human and murine cells. it targets a minimum epitope of 6 amino acids from the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GPX4
Purification MethodProtein G purified
UniProt Number
Molecular Weight~19 kDa observed; 19.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with Gpx4.

Western Blotting Analysis: 2 µg/mL of this antibody detected Glutathione Peroxidase 4 in lysate of HEK293 cells transfected with Gpx4.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF1969 04054839396632

Documentation

Anti-Glutathione Peroxidase 4 Antibody, clone Mab63-1 Certificates of Analysis

TitleLot Number
Anti-Glutathione Peroxidase 4, clone Mab63-1 - 3159546 3159546
Anti-Glutathione Peroxidase 4, clone Mab63-1 - 3757094 3757094
Anti-Glutathione Peroxidase 4, clone Mab63-1 - 3862806 3862806
Anti-Glutathione Peroxidase 4, clone Mab63-1 - 4079035 4079035
Anti-Glutathione Peroxidase 4, clone Mab63-1 - 4183790 4183790
Anti-Glutathione Peroxidase 4, clone Mab63-1 Monoclonal Antibody Q2959724
Anti-Glutathione Peroxidase 4, clone Mab63-1 Monoclonal Antibody 3060554