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AB5020 Anti-Glycine (Low Glutaraldehyde) Antibody

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AB5020
500 µL  2000 assays
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RIHCRbDiluted SerumPolyclonal Antibody
Description
Catalogue NumberAB5020
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Glycine (Low Glutaraldehyde) Antibody
References
Product Information
FormatDiluted Serum
Presentation IgG fraction in sterile 0.1M phosphate buffer. No preservative
Quality LevelMQ100
Applications
ApplicationDetect Glycine (Low Glutaraldehyde) using this Anti-Glycine (Low Glutaraldehyde) Antibody validated for use in IH.
Key Applications
  • Immunohistochemistry
Application NotesImmunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.5% - 2.5% glutaraldehyde for optimum detection.

*This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative). The minimum glutaraldehyde concentration for AB5020 is 0.05%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.

Optimal working dilutions must be determined by the end user.

DILUTION: Prepare enough of the AB5020 for your days use by diluting 100X with 1% GSPBT.
Biological Information
ImmunogenGlycine-glutaraldehyde-BSA
HostRabbit
SpecificityGlycine. The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable cross-reactivity (<1:1000) against glycine in peptides or proteins. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not freeze this stock.
Packaging Information
Material Size500 µL
Material Package2000 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB5020 04053252674358

Documentation

Anti-Glycine (Low Glutaraldehyde) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Glycine (Low Glutaraldehyde) Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-GLYCINE POLYCLONAL ANTIBODY - 2119989 2119989
RABBIT ANTI-GLYCINE POLYCLONAL ANTIBODY - 2421543 2421543
RABBIT ANTI-GLYCINE POLYCLONAL ANTIBODY - 2435071 2435071
RABBIT ANTI-GLYCINE - 2210315 2210315
RABBIT ANTI-GLYCINE - 2562456 2562456
RABBIT ANTI-GLYCINE - 3463566 3463566
RABBIT ANTI-GLYCINE - 3695882 3695882
RABBIT ANTI-GLYCINE - 3850579 3850579
RABBIT ANTI-GLYCINE -2544300 2544300
RABBIT ANTI-GLYCINE -2548030 2548030

References

Reference overviewPub Med ID
Optogenetic perturbation of preBötzinger complex inhibitory neurons modulates respiratory pattern.
Sherman, D; Worrell, JW; Cui, Y; Feldman, JL
Nature neuroscience  18  408-14  2015

Show Abstract
25643296 25643296
Retinal amino acid neurochemistry of the southern hemisphere lamprey, Geotria australis.
Nivison-Smith, L; Collin, SP; Zhu, Y; Ready, S; Acosta, ML; Hunt, DM; Potter, IC; Kalloniatis, M
PloS one  8  e58406  2013

Show Abstract
23516473 23516473
Recovery from paralysis in adult rats using embryonic stem cells.
Deepa M Deshpande, Yun-Sook Kim, Tara Martinez, Jessica Carmen, Sonny Dike, Irina Shats, Lee L Rubin, Jennifer Drummond, Chitra Krishnan, Ahmet Hoke, Nicholas Maragakis, Jeremy Shefner, Jeffrey D Rothstein, Douglas A Kerr
Annals of neurology  60  32-44  2006

Show Abstract
16802299 16802299