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MAB1271-I-100UG Anti-Golgi Zone Antibody, clone 371-4

Anti-Golgi Zone, clone 371-4, Cat. No. MAB1271-I, is a mouse monoclonal antibody that detects Golgi apparatus protein 1 and is tested for use in Immunocytochemistry.

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MAB1271-I-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMAB1271-I-100UG
DescriptionAnti-Golgi Zone Antibody, clone 371-4
Alternate Names
  • Golgi apparatus protein 1
  • CFR-1
  • Cysteine-rich fibroblast growth factor receptor
  • E-selectin ligand 1
  • ESL-1
  • Golgi sialoglycoprotein MG-160
Background InformationGolgi apparatus protein 1 (UniProt: Q92896; also known as CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand 1, ESL-1, Golgi sialoglycoprotein MG-160) is encoded by the GLG1 (also known as CFR1, ESL1, MG160) gene (Gene ID: 2734) in human. The Golgi apparatus plays a major role in transport, processing, and sorting of thousands of macromolecules in eukaryotic cells. It is readily recognizable because of its stacked membrane-coated cavities known as dictyosomes. The Golgi complex consists of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face and each of these regions contain various enzymes to selectively modify the macromolecules that pass through this complex. The Golgi complex is localized in the vicinity of the nucleus and is usually very large in secretory cells, where it fills most of the cytoplasm. Clone 371-4 selectively recognizes the Golgi complex in human cells. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Golgi Zone, clone 371-4, Cat. No. MAB1271-I, is a mouse monoclonal antibody that detects Golgi apparatus protein 1 and is tested for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesTested Application
Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Golgi apparatus protein 1 in A431cells.
Immunocytochemistry Analysis: A representative lot detected Golgi Zone in Immunocytochemistry applications (Endo, Y., et al. (2007). Cancer Sci.;98(10):1633-7; Strehlow, A.N., et al. (2007). Hum Mol Genet.;16(4):391-409; Oiso, S., et al. (2009). J Neurochem.;109(1):158-67; Leite, C., et al. (2014). PLoS One.;9(10):e111059).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenSU-DHL-1 human large cell lymphoma cells.
EpitopeUnknown
Clone371-4
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 371-4 is a mouse monoclonal antibody that detects Golgi apparatus.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Monkey.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GLG1
Purification MethodProtein G purified
UniProt Number
Molecular Weight134.55 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Golgi apparatus protein 1 in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB1271-I-100UG 04065267216621