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AB5220 Anti-Growth Associated Protein-43 (GAP-43) Antibody

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AB5220
100 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, Ch, BELISA, ICC, IHC, IP, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB5220
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Growth Associated Protein-43 (GAP-43) Antibody
Alternate Names
  • Neuromodulin
Background InformationGAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons.
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.01% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationAnti-Growth Associated Protein-43 (GAP-43) Antibody is an antibody against Growth Associated Protein-43 (GAP-43) for use in ELISA, IC, IH, IP & WB.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry:
1:1,000 dilution of a previous lot was used on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.

Immunocytochemistry:
1:1,000 dilution of a previous lot was used on Hippocampal neurons in culture and PC12 cells stimulated with NGF.

Immunoprecipitation:
1:100 dilution of a previous lot was used.

ELISA:
1:2,000 dilution of a previous lot was used.
Biological Information
ImmunogenRecombinant rat GAP-43 (complete sequence).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Growth Associated Protein-43 (GAP-43).
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Chicken
  • Bovine
Species Reactivity NoteReacts to a high degree with mouse. Reacts to a lesser degree with rat, human, bovine and chicken. Other species have not been tested.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This encoded protein is considered a crucial component of an effective regenerative response in the nervous system.
Gene Symbol
  • GAP43
  • PP46
  • B-50
Purification MethodAmmonium Sulfate Precipitation
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P17677 # This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons.
SIZE: 238 amino acids; 24803 Da
SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
PTM: Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
SIMILARITY: SwissProt: P17677 ## Belongs to the neuromodulin family. & Contains 1 IQ domain.
Molecular Weight23.6 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis: 1:500 dilution of this lot detected Growth Associated Protein 43 on 10 μg of Mouse brain lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for up to 6 months at -20ºC in undiluted aliquots from date of receipt.
Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB5220 04053252676963

Documentation

Anti-Growth Associated Protein-43 (GAP-43) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Growth Associated Protein-43 (GAP-43) Antibody Certificates of Analysis

TitleLot Number
Anti-Growth Associated Protein-43 (GAP-43) 2470399
Anti-Growth Associated Protein-43 (GAP-43) - 2428476 2428476
Anti-Growth Associated Protein-43 (GAP-43) - 2048775 2048775
Anti-Growth Associated Protein-43 (GAP-43) - 2167144 2167144
Anti-Growth Associated Protein-43 (GAP-43) - 2280291 2280291
Anti-Growth Associated Protein-43 (GAP-43) - 2362874 2362874
Anti-Growth Associated Protein-43 (GAP-43) - 3055414 3055414
Anti-Growth Associated Protein-43 (GAP-43) - 3245135 3245135
Anti-Growth Associated Protein-43 (GAP-43) - 3436010 3436010
Anti-Growth Associated Protein-43 (GAP-43) - 3510861 3510861

References

Reference overviewApplicationSpeciesPub Med ID
Contribution of Schwann Cells to Remyelination in a Naturally Occurring Canine Model of CNS Neuroinflammation.
Kegler, K; Spitzbarth, I; Imbschweiler, I; Wewetzer, K; Baumgärtner, W; Seehusen, F
PloS one  10  e0133916  2015

Show Abstract
26196511 26196511
Coupled local translation and degradation regulate growth cone collapse.
Deglincerti, A; Liu, Y; Colak, D; Hengst, U; Xu, G; Jaffrey, SR
Nature communications  6  6888  2015

Show Abstract
25901863 25901863
BDNF and NT4 play interchangeable roles in gustatory development.
Huang, T; Krimm, RF
Developmental biology  386  308-20  2014

Show Abstract
24378336 24378336
Promoting nerve regeneration in a neurotmesis rat model using poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly: in vitro and in vivo analysis.
Pereira, T; Gärtner, A; Amorim, I; Almeida, A; Caseiro, AR; Armada-da-Silva, PA; Amado, S; Fregnan, F; Varejão, AS; Santos, JD; Bartolo, PJ; Geuna, S; Luís, AL; Mauricio, AC
BioMed research international  2014  302659  2014

Show Abstract
25121094 25121094
Exuberant sprouting of sensory and sympathetic nerve fibers in nonhealed bone fractures and the generation and maintenance of chronic skeletal pain.
Chartier, SR; Thompson, ML; Longo, G; Fealk, MN; Majuta, LA; Mantyh, PW
Pain  155  2323-36  2014

Show Abstract
25196264 25196264
Neuroplasticity of sensory and sympathetic nerve fibers in a mouse model of a painful arthritic joint.
Ghilardi, JR; Freeman, KT; Jimenez-Andrade, JM; Coughlin, KA; Kaczmarska, MJ; Castaneda-Corral, G; Bloom, AP; Kuskowski, MA; Mantyh, PW
Arthritis and rheumatism  64  2223-32  2012

Show Abstract
22246649 22246649
Genomics of mature and immature olfactory sensory neurons.
Melissa D Nickell,Patrick Breheny,Arnold J Stromberg,Timothy S McClintock
The Journal of comparative neurology  520  2012

Show Abstract
22252456 22252456
Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain.
Lakshmana, MK; Hayes, CD; Bennett, SP; Bianchi, E; Reddy, KM; Koo, EH; Kang, DE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology  26  2072-83  2012

Show Abstract
22294787 22294787
Sensory and sympathetic nerve fibers undergo sprouting and neuroma formation in the painful arthritic joint of geriatric mice.
Jimenez-Andrade, JM; Mantyh, PW
Arthritis research & therapy  14  R101  2012

Show Abstract
22548760 22548760
Kinetics of neuronal contribution during the development of a contact allergic reaction.
Reem Altawil,Jonathan Lyström,Husameldin El-Nour
Archives of dermatological research  304  2012

Show Abstract
22198823 22198823

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Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies