Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABE364 Anti-H2A (H4R3me2s) Antibody, clone 6G1/1D3

Anti-H2A (H4R3me2s), clone 6G1/1D3, Cat. No. MABE364, is a highly specific mouse monoclonal antibody that targets H2A/H4R3me2s and has been tested in Dot Blot and Western Blotting.

Anti-H2A (H4R3me2s) Antibody, clone 6G1/1D3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HIST1H2AG, HIST1H2AG, H2AFP, HIST1H2AI, H2AFC UniProt Number: P0C0S8
View Products on Sigmaaldrich.com
MABE364
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HDB, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE364
DescriptionAnti-H2A (H4R3me2s) Antibody, clone 6G1/1D3
Alternate Names
  • Histone H2A type 1
  • H2A.1
  • Histone H2A/p+C10
  • Anti-H2A (H4R3me2s)
Background InformationHistone H2A type 1 (UniProt: P0C0S8; also known as H2A.1, Histone H2A/p) is encoded by the HIST1H2AG (also known as HIST1H2AG, H2AFP, HIST1H2AI, H2AFC) gene (Gene ID: 8329, 8320, 8332, 8336, 8369) in human. Histone H2A is one of four components of the core nucleosomal structure. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that serve important functions for regulation of gene expression, DNA repair, and chromatin. Histone H2A, in particular, undergoes acetylation on selected lysines, which may promote unfolding of chromatin and transcription. It can also be phosphorylated on selected serine residues, which mark mitosis and gene repression, or DNA damage. Arginine methylation is a less explored histone post-translational modification. It has been implicated in embryonic development, somatic transcriptional regulation, and cancer development. H2A arginine 3 methylation (R3me1, R3me2s, and R3me2a) is catalyzed by PRMT1 and PRMT5 in vitro. H2A methylation is shown to appear late in oogenesis and is most abundant in the laid egg. Arginine methylations are constrained to pre-mid blastula transition events in the embryo and are considered to be involved in the global transcriptional repression during development. H2A R3me2s is reported to be enriched on nucleosomes containing both active and repressive histone post-translational modifications in human A549 cells and Xenopus embryos. (Ref.: Wilczek, C et al. (2011). J Biol Chem. 286(49):42221-31; Wang, WL et al. (2014). BMC Epigenetics & Chromatin 7:22).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-H2A (H4R3me2s), clone 6G1/1D3, Cat. No. MABE364, is a highly specific mouse monoclonal antibody that targets H2A/H4R3me2s and has been tested in Dot Blot and Western Blotting.
Key Applications
  • Dot Blot
  • Western Blotting
Application NotesDot Blot Analysis: 1:500 dilution from a representative lot detected H2A (H4R3me2s) in H2A, H2B, and H4 dot blot.
Biological Information
ImmunogenKLH-conjugated linear symmetric di methyl R3 peptide.
Clone6G1/1D3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 6G1/1D3 specifically detects H2A/H4 arginine 3 symmetric dimethylation (H2A/H4R3me2) in acid extract of HeLa cells.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H2AG
  • HIST1H2AG
  • H2AFP
  • HIST1H2AI
  • H2AFC
Purification MethodProtein G purified
UniProt Number
Molecular Weight~15 kDa observed; 14.09 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell acid extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected H2A (H4R3me2s) in 10 µg of HeLa cell acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE364 04054839174513