Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

ABE2588-25UL Anti-H3Q5dopaminyl

Anti-H3Q5dopaminyl, Cat. No. ABE2588, is a rabbit polyclonal antibody that detects Histone H3 dopaminylated on glutamine 5 and is tested for use in Dot Blot, Peptide Inhibition Assay, and Western Blotting.

ABE2588-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABE2588-25UL
DescriptionAnti-H3Q5dopaminyl
Alternate Names
  • Dopaminylated Histone H3
  • H3Q5dopaminylated
Background InformationHistone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone 3.1 is also shown to undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. It has been reported that Histone H3 can also undergo dopaminylation ((H3Q5dop), which can potentiate the binding of adjacent histone post-translational modification interacting proteins and play a vital role in dopaminergic neuronal transcription. This rabbit polyclonal antibody specifically binds to Histone H3 dopaminylated on glutamine 5. (Ref.: Lepack, AE., et al (2020). Science. 368(6487); 197-201).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-H3Q5dopaminyl, Cat. No. ABE2588, is a rabbit polyclonal antibody that detects Histone H3 dopaminylated on glutamine 5 and is tested for use in Dot Blot, Peptide Inhibition Assay, and Western Blotting.
Key Applications
  • Dot Blot
  • Peptide Inhibition Assay
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:200 dilution from a representative lot detected H3Q5dopaminyl in human ventral tegmental area (Courtesy of Ian Maze Lab; Icahn School of Medicine at Mount Sinai, New York).

Dot Blot Analysis: A representative lot detected H3Q5dopaminyl in Dot Blot applications (Lepack, AE., et al. (2020). Science. 368(6487); 197 201).

Peptide Inhibition Assay: A representative lot detected H3Q5dopaminyl in Peptide Inhibition Assay applications ( Lepack, AE., et al. (2020). Science. 368(6487); 197 201).

Western Blotting Analysis: A representative lot detected H3Q5dopaminyl in Western Blotting applications. (Lepack, AE., et al. (2020). Science. 368(6487); 197 201).

Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected H3Q5dopaminyl in Unmodified H3Q5 peptide vs. H3Q5dopaminyl peptide (Courtesy of Ian Maze Lab; Icahn School of Medicine at Mount Sinai, New York).



Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3 with dopaminylated glutamine 5.
EpitopeN-terminus
Concentration0.03 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Histone H3 dopaminylated on glutamine 5.
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~17 kDa observed; 15.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant H3 dopaminyated on glutamine 5.

Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant Histone H3 dopaminylated on glutamine 5 with 2.5 mM dopamine, but not with one treated with transglutaminase 2 (0.5 mg).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE2588-25UL 04061842414895

Documentation

Anti-H3Q5dopaminyl SDS

Title

Safety Data Sheet (SDS) 

Anti-H3Q5dopaminyl Certificates of Analysis

TitleLot Number
Anti-H3Q5dopaminyl - 3684698 3684698
Anti-H3Q5dopaminyl - 3689468 3689468
Anti-H3Q5dopaminyl - 3695325 3695325
Anti-H3Q5dopaminyl - 3700374 3700374
Anti-H3Q5dopaminyl - 3715675 3715675
Anti-H3Q5dopaminyl - 3719550 3719550
Anti-H3Q5dopaminyl - 3720089 3720089
Anti-H3Q5dopaminyl - 3726522 3726522
Anti-H3Q5dopaminyl - 3729955 3729955
Anti-H3Q5dopaminyl - 3734501 3734501
Show more

References

Reference overviewPub Med ID
Dopaminylation of histone H3 in ventral tegmental area regulates cocaine seeking
Ashley E Lepack 1 , Craig T Werner 2 , Andrew F Stewart 1 , Sasha L Fulton 1 , Ping Zhong 3 , Lorna A Farrelly 1 , Alexander C W Smith 1 , Aarthi Ramakrishnan 1 , Yang Lyu 1 , Ryan M Bastle 1 , Jennifer A Martin 2 , Swarup Mitra 2 , Richard M O'Connor 1 , Zi-Jun Wang 3 , Henrik Molina 4 , Gustavo Turecki 5 , Li Shen 1 , Zhen Yan 3 , Erin S Calipari 6 , David M Dietz 2 , Paul J Kenny 1 , Ian Maze
Science  368(6487)  197-201  2020

Show Abstract 		32273471 32273471