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04-902 Sigma-AldrichAnti-HA Tag Antibody, clone DW2, rabbit monoclonal

Anti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Epitope Tags & General Use Research Sub-Category: Epitope Tags

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
Vrt	WB	Rb	Culture Supernatant	Monoclonal Antibody

Description
Catalogue Number	04-902
Replaces	05-902
Description	Anti-HA Tag Antibody, clone DW2, rabbit monoclonal
Background Information	Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

References

Product Information
Format	Culture Supernatant
Control	HA-tagged WNT-1 protein.
Presentation	Cultured supernatant in 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.
Key Applications	Western Blotting
Application Notes	Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.

Biological Information
Immunogen	KLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.
Clone	DW2
Host	Rabbit
Specificity	Recognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.
Isotype	IgG
Species Reactivity	Vertebrates
Species Reactivity Note	Most common vertebrate species tested. Reactivity with other species has not been confirmed.
Antibody Type	Monoclonal Antibody
Molecular Weight	Various

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Western Blot Analysis Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Western Blot Analysis
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
04-902	04053252738203

Documentation

Anti-HA Tag Antibody, clone DW2, rabbit monoclonal SDS

Title
Safety Data Sheet (SDS)

Anti-HA Tag Antibody, clone DW2, rabbit monoclonal Certificates of Analysis

Title	Lot Number
Anti-HA Tag, clone DW2 - 2444134	2444134
Anti-HA Tag, clone DW2 - 2299155	2299155
Anti-HA Tag, clone DW2 - 2957992	2957992
Anti-HA Tag, clone DW2 - 3593758	3593758
Anti-HA Tag, clone DW2 - 3886618	3886618
Anti-HA Tag, clone DW2 - 4045806	4045806
Anti-HA Tag, clone DW2 - DAM1561709	DAM1561709
Anti-HA Tag, clone DW2 -2660175	2660175
Anti-HA Tag, clone DW2 -2683839	2683839
Anti-HA Tag, clone DW2 -2819147	2819147

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