Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABE1122 Anti-HOIP (RNF31) Antibody, clone 1CB2

Detect HOIP using this mouse monoclonal Anti-HOIP (RNF31), clone 1CB2 Antibody, Cat. No. MABE1122, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-HOIP (RNF31) Antibody, clone 1CB2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: RNF31, ZIBRA UniProt Number: Q96EP0
View Products on Sigmaaldrich.com
MABE1122
100 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1122
DescriptionAnti-HOIP (RNF31) Antibody, clone 1CB2
Alternate Names
  • E3 ubiquitin-protein ligase RNF31
  • HOIL-1-interacting protein
  • HOIP
  • RING finger protein 31
  • Zinc in-between-RING-finger ubiquitin-associated domain protein
Background InformationE3 ubiquitin-protein ligase RNF31 (UniProt Q96EP0; also known as HOIL-1-interacting protein, HOIP, RING finger protein 31, Zinc in-between-RING-finger ubiquitin-associated domain protein) is encoded by the RNF31 (also known as ZIBRA) gene (Gene ID 55072) in human. HOIP is a RING-between-RING (RBR) family E3 ubiquitin ligase and the only enzyme known to catalyze linear-ubiquitin-chain conjugation. HOIP functions as the primary E3 Ub ligase of the immune signaling regulatory complex LUBAC (linear-ubiquitin-chain assembly complex) composed of heme-oxidized IRP2 ubiquitin ligase-1 (HOIL-1L), HOIP, and SHANK-associated RH domain interactor (Sharpin). RBR ligases often exist in autoinhibited conformations and require protein-protein interactions or posttranslational modifications to induce a conformational change for activation. Disruption of HOIP and HOIL-1L interaction in activated B cell-like (ABC) subtype of diffuse large B-cell lymphoma (DLBCL) induces DLBCL death as a result of decreased NF-κB activation due to HOIP inactivation. On the other hand, HOIP K1056R mutant is resistant to inactivation by TLR4 stimulation-induced HOIP K1056 ubiquitination. Human HOIP is a 1072-a.a. protein that contains a polyUb-binding region (a.a. 1-485), a ubiquitin-associated or UBA domain (a.a. 564-615) that mediates HOIL-1L/RBCK1 interactioin, and a C-terminal linear-ubiquitin-chain-determining domain or LDD domain (a.a. 910-1072). In addition, HOIP conatins three RanBP2-type Zinc fingers (a.a. 299-329, 350-379, 409-438), two RING-type Zinc fingers (a.a. 699-747 and 860-909), and one IBR-type Zinc fingers (a.a. 779-841).
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect HOIP using this mouse monoclonal Anti-HOIP (RNF31), clone 1CB2 Antibody, Cat. No. MABE1122, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:10 dilution from a representative lot detected HOIP (RNF31) in human uterus tissue section.

Western Blotting Analysis: 1 µg/mL from a representative lot detected endogenous HOIP (RNF31) in 60 µg of lysate from HEK293T and wild-type, but not HOIP-knockout, Jurkat cells, as well as the overexpression of an myc-tagged human HOIP in transfected HEK293T cells (Courtesy of Professor Kazuhiro Iwai, Kyoto University School of Medicine, Japan).

Western Blotting Analysis: 1 µg/mL from a representative lot detected endogenous HOIP (RNF31) in 60 µg of human (Jurkat, K562, U937), but not mouse (MEF & Bal17.2) cell lysates, as well as the overexpression of an myc-tagged human HOIP in transfected HEK293T cells (Courtesy of Professor Kazuhiro Iwai, Kyoto University School of Medicine, Japan).
Biological Information
ImmunogenGST-tagged recombinant human HOIP (RNF31) LDD domain.
Clone1CB2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1CB2 detected a ~120 kDa target band only in parental, but not HOIP-knock, Jurkat cells. Clone 1CB2 targets an epitope within the C-terminal LDD domain present in all three human HOIP (RNF31) spliced isoforms reported by UniProt (Q96EP0).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Not Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RNF31
  • ZIBRA
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~120 kDa observed. 119.7/57.63/102.5 kDa (isoform 1/2/3) calculated. Target band often appears as a doublet with the upper band representing ubiquitinated HOIP. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceImmunohistochemistry Analysis: A 1:10 dilution of this antibody detected HOIP in human adrenal gland tissue section.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1122 04054839057236