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MABC1139-100UG Anti-HPCAL1 Antibody, clone 1E10

Detects Hippocalcin-like protein 1 using this mouse monoclonal Anti-HPCAL1, clone 1E10, Cat. No. MABC1139, tested for use in Western Blotting and Immunoprecipitation.

Anti-HPCAL1 Antibody, clone 1E10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: HPCAL1, BDR1 UniProt Number: P37235
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MABC1139-100UG
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1139-100UG
DescriptionAnti-HPCAL1 Antibody, clone 1E10
Alternate Names
  • Hippocalcin-like protein 1
  • Calcium-binding protein BDR-1
  • HLP2
  • Visinin-like protein 3
  • VILIP-3
Background InformationHippocalcin-like protein 1 (UniProt: P37235; also known as Calcium-binding protein BDR-1, HLP2, Visinin-like protein 3, VILIP-3) is encoded by the HPCAL1 (also known a BDR1) gene (Gene ID: 3241) in human. HPCAL1 is a lipid-anchor membrane protein of the visinin-like subfamily of neural calcium sensor and is involved in the calcium-dependent regulation of rhodopsin phosphorylation. It is structurally related to Ca2+-myristoyl switch proteins that contain four EF-hand motifs and a covalently attached N-terminal myristoyl group. HPCAL1 is also expressed in Purkinje cells of the cerebellum and hippocampus where it is believed to regulate synaptic plasticity relevant for learning and memory. Clone 1E10 specifically detects denatured HPCAL1 protein that is endogenously expressed in several pancreatic cell lines. (Ref.: Chen, Y., et al. (2014). Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 33(1); 20-27; Li, C., et al. (2016). PLoS One 11(11); e0165921).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
Applications
ApplicationDetects Hippocalcin-like protein 1 using this mouse monoclonal Anti-HPCAL1, clone 1E10, Cat. No. MABC1139, tested for use in Western Blotting and Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: A representative lot detected HPCAL1 in Western Blotting applications (Chen, Y., et. al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7).

Immunoprecipitation Analysis: A representative lot detected HPCAL1 in Immunoprecipitation applications (Chen, Y., et. al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7).
Biological Information
ImmunogenPurified native and denatured human HPCAL1.
Clone1E10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1E10 specifically detects Hippocalcin-like protein 1 (HPCAL1) in human cells.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HPCAL1
  • BDR1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~22 kDa observed; 22.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Panc-1 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected HPCAL1 in Panc-1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC1139-100UG 04054839529436