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MABT1438-100UL Anti-HYAL1 Antibody, clone 1D10

Anti-HYAL1, clone 1D10, Cat. No. MABT1438, is a mouse monoclonal antibody that detects Hyaluronidase-1 and is tested for use in Immunohistochemistry, Western Blotting, and Western Blotting using knockout lysate.

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MABT1438-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABT1438-100UL
DescriptionAnti-HYAL1 Antibody, clone 1D10
Alternate Names
  • Hyaluronidase-1
  • EC:3.2.1.35
  • Hyal-1
  • Hyaluronoglucosaminidase-1
  • Lung carcinoma protein 1
  • LuCa-1
Background InformationHyaluronidase-1 (UniProt: Q12794; also known as EC:3.2.1.35, Hyal-1, Hyaluronoglucosaminidase-1, Lung carcinoma protein 1, LuCa-1) is encoded by the HYAL1 (also known as LUCA1) gene (Gene ID: 3373) in human. Hyaluronidases (HYAL) catalyze the breakdown of hyaluronic acid at the β1-4 linkage between N-acetyl-β-D-glucosamine and D-glucuronic acid to generate smaller oligosaccharides. HYAL is an acid hydrolase with a pH optimum of ~ 3.8. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that is mainly present in the lysosomal compartment. In murine macrophages, HYAL1 is reportedly trafficked to the lysosomes by a Man-6-P-independent secretion/recapture mechanism mediated by the mannose receptor. Its expression is higher in the liver, kidney, and heart and weak expression is observed in lung, placenta, and skeletal muscle. It has also been detected in plasma and in urine. HYAL-1 is also responsible for the production of angiogenic HA fragments. Its presence in urine and change in expression level in bladder and prostate makes in a marker of bladder and prostate cancers. Significantly higher expression of HYA1 is observed in advanced stages of bladder cancer. Inactivating mutations of HYAL1 gene have been linked to mucopolysaccharidosis IX that is characterized by diminished stature and periarticular soft tissue masses with 40 to 90-fold higher levels of hyaluronic acid than in normal serum. (Ref.: Puissant, E., et al. (2014). Traffic. 15(5); 500-515; Chao, KL., et al. (2007). Biochemistry. 46(23); 6911-6920).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-HYAL1, clone 1D10, Cat. No. MABT1438, is a mouse monoclonal antibody that detects Hyaluronidase-1 and is tested for use in Immunohistochemistry, Western Blotting, and Western Blotting using knockout lysate.
Key Applications
  • Western Blotting
  • Western Blot Using KO Lysates
  • Immunohistochemistry
Application NotesTested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected HYAL1 in Mouse liver tissue lysate.

Western Blot using KO lysates: A 1:500 dilution from a representative lot detected HYAL1 in lysate from wild-type liver tissue, but not in mouse liver with HYAL1 knockout. (Data courtesy of Dr. Barbara Triggs-Raine, Ph.D., University of Manitoba, Canada).

Western Blotting Analysis: A representative lot detected HYAL1 in Western Blotting application. (Shuttleworth, T., et al. (2002). J Biol Chem. 277(25):23008-18; Martin, D.C., et al. (2008). Hum Mol Genet. 17(13):1904-15; Hemming, R., et al. (2008). Glycobiology. 18(4):280-9; Puissant, E., et al. (2014). Traffic. 15(5):500-15).

Immunohistochemistry Applications: A representative lot detected HYAL1 in Immunohistochemistry application. (de Sa, V.K., et al. (2015). Braz J Med Biol Res. 48(11):1039-47).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to the first 366 amino acids from human Hyaluronidase-1.
EpitopeUnknown
Clone1D10
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1D10 is a mouse monoclonal antibody that detects Hyaluronidase-1.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Hamster
  • Human
Species Reactivity NoteHuman, Hamster, Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HYAL1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~52 kDa observed; 48.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse kidney tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Hyaluronidase-1 in Mouse kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT1438-100UL 04065266876161