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AB5714 Anti-Hey 1/HRT1 Antibody

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AB5714
100 µg  
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Overview

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5714
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Hey 1/HRT1 Antibody
Product Information
FormatAffinity Purified
PresentationPrecipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-Hey 1/HRT1 Antibody detects level of Hey 1/HRT1 & has been published & validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern blot: 1:200-1:1,000 using ECL.

Immunohistochemistry: 1:200-1:1,000.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide, amino acids 207-220 of human Hey 1 / HRT1.
HostRabbit
SpecificityRecognizes Hey 1 / HRT1, a Helix-loop-helix class of transcription factor.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants.
Gene Symbol
  • HEY1
  • CHF-2
  • MGC1274
  • CHF2
  • HERP2
  • HRT1
  • hHRT1
  • HRT-1
  • HESR1
  • OAF1
  • HESR-1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9Y5J3 # Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'- CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
SIZE: 304 amino acids; 32613 Da
SUBUNIT: Self-associates (By similarity). Interacts with HES1 and HEYL (By similarity). Interacts with HDAC1, NCOR1 and SIN3A (By similarity). Interacts with GATA4 and GATA6 (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity).
TISSUE SPECIFICITY: Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
SIMILARITY: SwissProt: Q9Y5J3 ## Belongs to the HEY family. & Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 Orange domain.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.


For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalog Number GTIN
AB5714 04053252468407