Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

ABE1348 Anti-Histone H2A.Z Antibody (C-term)

This Anti-Histone H2A.Z Antibody (C-term) is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Immunocytochemistry and ELISA for the detection of Histone H2A.Z (C-term).

Anti-Histone H2A.Z Antibody (C-term) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H2AFZ, H2AZ UniProt Number: P0C0S5
View Products on Sigmaaldrich.com
ABE1348
50 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ChIP, ChIP-seq, ICC, ELISARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1348
DescriptionAnti-Histone H2A.Z Antibody (C-term)
Alternate Names
  • Histone H2A.Z
  • H2A/z
Background InformationH2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
References
Product Information
FormatAffinity Purified
PresentationAffinity purified rabbit polyclonal in PBS containing 0.05% azide and 0.05% ProClin 300.
Quality LevelMQ100
Applications
ApplicationThis Anti-Histone H2A.Z Antibody (C-term) is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Immunocytochemistry and ELISA for the detection of Histone H2A.Z (C-term).
Key Applications
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
  • ChIP-seq
  • Immunocytochemistry
  • ELISA
Application NotesChromatin Immunoprecipitation (ChIP): A representative lot of this antibody (1μg per reaction) was used for ChIP using chromatin from HeLa and K562 cells.

ChIP-seq: A representative lot of this antibody (0.5 μg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis.

Western Blotting Analysis: A 1:2,000 dilution of a represenative lot of this antibody detected histone H2A.Z in acid extracted HeLa cell lysate.

Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H2A.Z in HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to region the near C-terminus of Human Histone H2A.Z.
EpitopeNear C-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes histone H2A.Z
Species Reactivity
  • Human
Species Reactivity NoteHuman, wide range expected
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • H2AFZ
  • H2AZ
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~16 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting using recombinant histones

Western Blotting Analysis: A 1.5 µg/mL dilution of this antibody detected recombinant Histone H2A.Z, but not histones H1, H2A,H2A.X, H3, or H4.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE1348 04055977073195

Documentation

Anti-Histone H2A.Z Antibody (C-term) SDS

Title

Safety Data Sheet (SDS) 

Anti-Histone H2A.Z Antibody (C-term) Certificates of Analysis

TitleLot Number
Anti-Histone H2A.Z (C-term) Q2464803
Anti-Histone H2A.Z (C-term) - 3226591 3226591
Anti-Histone H2A.Z (C-term) - 3255344 3255344
Anti-Histone H2A.Z (C-term) - 3275084 3275084
Anti-Histone H2A.Z (C-term) - 3310689 3310689
Anti-Histone H2A.Z (C-term) - 3439804 3439804
Anti-Histone H2A.Z (C-term) - 3510291 3510291
Anti-Histone H2A.Z (C-term) - 3574584 3574584
Anti-Histone H2A.Z (C-term) - 3596866 3596866
Anti-Histone H2A.Z (C-term) - 3689960 3689960
Show more