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ABE215 Anti-Histone Macro H2A.1 Antibody

Anti-Histone Macro H2A.1 Antibody is a Rabbit Polyclonal Antibody for detection of Histone Macro H2A.1 also known as H2A histone family member Y, histone macroH2A1.2 & has been validated in WB, ICC.

Anti-Histone Macro H2A.1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H2A.y, H2AF12M, H2AFJ, H2A/y, MACROH2A1.1, mH2A, MACROH2A UniProt Number: Q9QZQ8
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ABE215
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE215
Replaces07-219
DescriptionAnti-Histone Macro H2A.1 Antibody
Alternate Names
  • Core histone macro-H2A.1
  • Histone macroH2A1.2
  • mH2A1
  • H2A.y
  • H2A/y
Background InformationHistone MacroH2A (mH2A) is a variant histone also known as H2AFY. mH2A is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. This histone variant is known to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. In contrast to most histone variants containing only subtle changes, mH2A contains a large non-histone domain (the macro domain) on its C-terminus, resulting in a histone approximately three times the size of conventional H2A. There are 3 isoforms of mH2A, two of which (mH2A1.1, mH2A1.2 ) are formed by alternative splicing and another (mH2A2) from a separate gene. There are two phosphorylation sites within the basic hinge region of mH2A: Thr128 and Ser137. Phospho-Ser137 is enriched in mitosis, implicating mH2A in the regulation of posttranslational modifications throughout the cell cycle.
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
Control
  • HeLa acid extract
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Histone Macro H2A.1 Antibody is a Rabbit Polyclonal Antibody for detection of Histone Macro H2A.1 also known as H2A histone family member Y, histone macroH2A1.2 & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone MacroH2A.1 in NIH/3T3, A431, and HeLa cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to mcro domain of mouse Histone MacroH2A.1.
EpitopeMacro domain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Histone MacroH2A.1 at the macro domain.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • H2A.y
  • H2AF12M
  • H2AFJ
  • H2A/y
  • MACROH2A1.1
  • mH2A
  • MACROH2A
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.

SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 (By similarity).

SUBCELLULAR LOCATION: Nucleus. Chromosome. Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. In quiescent lymphocytes, associated with centromeric constitutive heterochromatin.

TISSUE SPECIFICTY: Ubiquitously expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level). In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, present in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla.

PTM: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin (By similarity).

SEQUENCE SIMILARITIES: Contains 1 histone H2A domain.

Contains 1 Macro domain.
Molecular Weight~ 40 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 0.2 µg/mL of this antibody detected histone MacroH2A.1 on 10 µg of HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE215 04053252756566

Documentation

Anti-Histone Macro H2A.1 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Histone Macro H2A.1 Antibody Certificates of Analysis

TitleLot Number
Anti-Histone macroH2A.1 - 2458008 2458008
Anti-Histone macroH2A.1 - 2295491 2295491
Anti-Histone macroH2A.1 - 3249946 3249946
Anti-Histone macroH2A.1 - 3434609 3434609
Anti-Histone macroH2A.1 - 3473512 3473512
Anti-Histone macroH2A.1 - 3494042 3494042
Anti-Histone macroH2A.1 - 3863963 3863963
Anti-Histone macroH2A.1 - NRG1950703 NRG1950703
Anti-Histone macroH2A.1 -2700262 2700262
Anti-Histone macroH2A.1 -2776229 2776229
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Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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