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386023 Anti-Hsp47 Mouse mAb (M16.10A1)

Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).

Anti-Hsp47 Mouse mAb (M16.10A1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Colligin

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386023
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
B, Ca, Ch, Gp, Ht, H, Mk, M, Porcine, R, ShMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
386023-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes the ~47 kDa Hsp47 protein.
Catalogue Number386023
Brand Family Calbiochem®
SynonymsAnti-Colligin
Application Data
Detection of mouse Hsp47 by immunoblotting. Sample: Whole cell lysate from 3T3 cells. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:1000). Detection: chemiluminescence.

(left) Detection of rat Hsp47 by staining paraffin sections. Sample: Paraffin-embedded rat carotoid artery after balloon injury to the artery. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023). Detection: colorimetric (DAB). (right) Detection of human Hsp47 by staining paraffin sections. Sample: Human breast cancer tissue. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:50). Detection: colorimetric (DAB).
References
ReferencesKuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res. 179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol. 105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Positive control3T3 cells, Human breast cancer tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme Immunoassay
Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesELISA (see comments)
Enzyme Immunoassay (see comments)
Immunoblotting (1 µg/ml, colorimetric)
Immunocytochemistry (1 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (see comments)
Application CommentsThis antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.
Biological Information
ImmunogenHsp47 from L6 cells
ImmunogenRat
CloneM16.10A1
HostMouse
IsotypeIgG2b
Species Reactivity
  • Bovine
  • Canine
  • Chicken
  • Guinea Pig
  • Hamster
  • Human
  • Monkey
  • Mouse
  • Porcine
  • Rat
  • Sheep
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
386023-50UG 04055977189872

Documentation

Anti-Hsp47 Mouse mAb (M16.10A1) SDS

Title

Safety Data Sheet (SDS) 

Anti-Hsp47 Mouse mAb (M16.10A1) Certificates of Analysis

TitleLot Number
386023

References

Reference overview
Kuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res. 179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol. 105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-August-2007 RFH
SynonymsAnti-Colligin
ApplicationELISA (see comments)
Enzyme Immunoassay (see comments)
Immunoblotting (1 µg/ml, colorimetric)
Immunocytochemistry (1 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (see comments)
Application Data
Detection of mouse Hsp47 by immunoblotting. Sample: Whole cell lysate from 3T3 cells. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:1000). Detection: chemiluminescence.

(left) Detection of rat Hsp47 by staining paraffin sections. Sample: Paraffin-embedded rat carotoid artery after balloon injury to the artery. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023). Detection: colorimetric (DAB). (right) Detection of human Hsp47 by staining paraffin sections. Sample: Human breast cancer tissue. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:50). Detection: colorimetric (DAB).
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.
BackgroundHsp47 is a unique heat shock protein with collagen binding ability. A member of the serpin family of proteins, Hsp47 localizes within the ER due to an RDEL sequence, a variant of the KDEL sequence. Hsp47 is thought to bind procollagen immediately after it co-translationally enters the ER, and it dissociates from procollagen before its secretion. The ability of Hsp47 to bind collagen I and collagen IV suggests it may function as a molecular chaperone for these proteins.
HostMouse
Immunogen speciesRat
ImmunogenHsp47 from L6 cells
CloneM16.10A1
IsotypeIgG2b
Speciesbovine, canine, chicken, guinea pig, hamster, human, monkey, mouse, porcine, rat, sheep
Positive control3T3 cells, Human breast cancer tissue
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res. 179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol. 105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.