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05-172-AF488 Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor® 488 Conjugate

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor® 488 Conjugate is an antibody against IGF-I for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: IGF1, IBP1 UniProt Number: P05019
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05-172-AF488
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, F, H, M, RICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number05-172-AF488
DescriptionAnti-IGF-I Antibody, clone Sm1.2, Alexa Fluor® 488 Conjugate
Alternate Names
  • Insulin-like growth factor I
  • IGF-I
  • Mechano growth factor
  • MGF
  • Somatomedin-C
Background InformationInsulin-like growth factor I (UniProt P05019; also known as IGF-I, Somatomedin-C) is encoded by the IGF1 (also known as IGFI, MGF) gene (Gene ID 3479) in human. Human insulin-like growth factor-I (IGF-I) is a single chain 70 amino acid polypeptide that shares structural homology with insulin and human IGF-II. IGF-I plays a critical role in growth, differentiation and development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation. IGF-I is a regulator of metabolism and is also implicated in the pathophysiology of cancer, diabetes and obesity. Upon binding the tetrameric (alpha:beta)2 IGF-I receptor, the receptor alpha chains induce the tyrosine autophosphorylation of the beta chains, which in turn trigger cell type-specific downstream signaling cascade of events.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-IGF-I Antibody, clone Sm1.2, Alexa Fluor® 488 Conjugate is an antibody against IGF-I for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IGF-I in HeLa and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-172) is shown to be suitable also for Western blotting, immunohistochemistry, immunoprecipitation, and neutralization applications.
Biological Information
ImmunogenHighly purified human plasma insulin-like growth factor I (IGF-I; Somatomedin C; Sm-C) conjugated to mouse albumin (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522). Action of somatomedins on cell growth: Effect of selective neutralisation of somatomedin C (IGF-I) with a monoclonal antibody. In: Raiti, S., Tolman, and R.A. (eds.), Human Growth Hormone. pp. 585–599. Plenum Publishing Corp., New York, NY).
CloneSm1.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityIGF-I (Russel, W.E., (1984). Proc. Natl. Acad. Sci. U. S. A. 81(8):2389-2392). 40% cross reactivity to IGF-II (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522).
IsotypeIgG1κ
Species Reactivity
  • Chicken
  • Fish
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IGF1
  • IBP1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IGF-I in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-172-AF488 04055977311921