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AP1145 Anti-IL13RA2 Mouse mAb (2E10)

This Anti-IL13RA2 Mouse mAb (2E10) is validated for use in ELISA, Immunoblotting for the detection of IL13RA2.

Anti-IL13RA2 Mouse mAb (2E10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Interleukin-13 Receptor αChain 2

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AP1145
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
AP1145-100UG 100 μg
Description
OverviewRecognizes the ~50 kDa IL13RA2 protein in HeLa cells.
Catalogue NumberAP1145
Brand Family Calbiochem®
SynonymsAnti-Interleukin-13 Receptor αChain 2
Application Data
Detection of human IL13RA2 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-IL13RA2 Mouse mAb (2E10) (Cat. No. AP1145) (1-5 µg/ml). Detection: chemiluminesence.
References
ReferencesZeis, T., et al. 2008. Brain 131 288.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 27-127 of human IL13RA2, expressed as a GST fusion protein
Clone2E10
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AP1145-100UG 04055977227871

Documentation

Anti-IL13RA2 Mouse mAb (2E10) SDS

Title

Safety Data Sheet (SDS) 

Anti-IL13RA2 Mouse mAb (2E10) Certificates of Analysis

TitleLot Number
AP1145

References

Reference overview
Zeis, T., et al. 2008. Brain 131 288.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-October-2010 JLN
SynonymsAnti-Interleukin-13 Receptor αChain 2
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human IL13RA2 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-IL13RA2 Mouse mAb (2E10) (Cat. No. AP1145) (1-5 µg/ml). Detection: chemiluminesence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa IL13RA2 protein.
BackgroundIL13RA2 is closely related to IL13RA1, a subunit of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks a cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 27-127 of human IL13RA2, expressed as a GST fusion protein
Clone2E10
IsotypeIgG2a
Specieshuman
Positive controlHeLa cells
FormLiquid
FormulationIn PBS, pH 7.2.
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesZeis, T., et al. 2008. Brain 131 288.