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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABE1328 Sigma-AldrichAnti-INK4a (p16) Antibody, clone 13H4.1

Anti-INK4a (p16), clone 13H4.1 Antibody, Cat. No. MABE1451, is a highly specific mouse monoclonal antibody that targets INK4a (p16) and has been tested in Immunocytochemistry and Western Blotting.

Anti-INK4a (p16) Antibody, clone 13H4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Epigenetics & Nuclear Function Gene Symbol: CDKN2A, CDKN2, MTS1 UniProt Number: P42771

Replacement Information
Replacement Information	04-239

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IH(P)	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE1328
Description	Anti-INK4a (p16) Antibody, clone 13H4.1
Alternate Names	Cyclin-dependent kinase inhibitor 2A CDK4I Multiple tumor suppressor 1 MTS-1 p16-INK4 p16-INK4a p16INK4A
Background Information	Cyclin-dependent kinase inhibitor 2A (UniProt P42771; also known as CDK4I, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, MTS-1, p16-INK4, p16-INK4a, p16INK4A) is encoded by the CDKN2A (also known as CDKN2, MTS1) gene (Gene ID 1029) in human. The INK and CIP/KIP families of cyclin dependent kinase inhibitors (CDKis) protect cells from oncogenic signals-initiated cellular transformation. INK4a and INK4b (encoded by the CDKN2A and CDKN2B gene, respectively) constitute the INK4 subfamily of CDKis. INK4a and INK4b are induced by oncogenic Ras and are involved in cell cycle G1 arrest and cellular senescence. Inactivation of CDKN2A and CDKN2B are found in a wide variety of human malignancies and murine tumor models.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-INK4a (p16), clone 13H4.1 Antibody, Cat. No. MABE1451, is a highly specific mouse monoclonal antibody that targets INK4a (p16) and has been tested in Immunocytochemistry and Western Blotting.
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p16INK4a in human kidney and small intestine tissue sections.

Biological Information
Immunogen	GST-tagged recombinant human p16INK4a internal fragment.
Clone	13H4.1
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 13H4.1 epitope lies within a region present in p16INK4a and the spliced isoforms 2 and 5 (UniProt P42771-1, P42771-2, and P42771-4 respectively), but absent from the spliced isoform 3 (p12; UniProt P42771-3), p14ARF/p19ARF or smARF (UniProt Q8N726-1 and Q8N726-2).
Isotype	IgG2bκ
Species Reactivity	Human
Species Reactivity Note	Human.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000068
Gene Symbol	CDKN2A CDKN2 MTS1
Purification Method	Protein G purified.
UniProt Number	P42771
Molecular Weight	~16 kDa observed. 16.53 kDa (isoform 1; p16INK4a), 11.22 kDa (isoform 2), and 17.88 (isoform 5; p16gamma) calculated. Uncharacterized bands may be observed in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected p16INK4a in 10 µg of HEK293 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	200 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
MABE1328	04054839034473

Title	Lot Number
Anti-INK4a (p16), clone 13H4.1 - 3209171	3209171
Anti-INK4a (p16), clone 13H4.1 - 3379907	3379907
Anti-INK4a (p16), clone 13H4.1 - 3823685	3823685
Anti-INK4a (p16), clone 13H4.1 -Q2724506	Q2724506
Anti-INK4a (p16), clone 13H4.1 Monoclonal Antibody	2916002
Anti-INK4a (p16), clone 13H4.1 Monoclonal Antibody	3123352

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