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AB5882-200UL Anti-IP3 Receptor 1 Antibody

This Anti-IP3 Receptor 1 Antibody is validated for use in WB for the detection of IP3 Receptor 1.

Anti-IP3 Receptor 1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: GPCR, cAMP/cGMP & Calcium Signaling Gene Symbol: ITPR1, IP3R1, SCA15, InsP3R1, IP3R, INSP3R1, Insp3r1 UniProt Number: Q14643
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AB5882-200UL
200 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5882-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-IP3 Receptor 1 Antibody
Alternate Names
  • Inositol 1,4,5-Trisphosphate Receptor 1
  • ITPR1
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationLyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg fo
Quality LevelMQ100
Applications
ApplicationThis Anti-IP3 Receptor 1 Antibody is validated for use in WB for the detection of IP3 Receptor 1.
Key Applications
  • Western Blotting
Application NotesWestern blot: 1:200-1:1,000 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide corresponding amino acids 2732-2750 of rat IP3R1 (Accession number P29994). It is expected that the antibody will also react with mouse and human due to identical sequence homology.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes IP3 Receptor 1. The epitope does not share homology with any other known proteins.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ITPR1
  • IP3R1
  • SCA15
  • InsP3R1
  • IP3R
  • INSP3R1
  • Insp3r1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q14643 # Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5- trisphosphate.
SIZE: 2758 amino acids; 313945 Da
SUBUNIT: Homotetramer. Interacts with TRPC4. The PPXXF motif binds HOM1, HOM2 and HOM3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3. Interacts with TXNDC4 in a pH-, redox state- and calcium- dependent manner which results in the inhibition the calcium channel activity. The strength of this interaction inversely correlates with calcium concentration. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Interacts with AHCYL1 (By similarity). Interacts with MRVI1.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed.
DOMAIN: SwissProt: Q14643 The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand- binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
PTM: Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels (By similarity). & Phosphorylated on tyrosine residues.
DISEASE: SwissProt: Q14643 # Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of autosomal dominant cerebellar ataxias (ADCA). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements. Spinocerebellar ataxia is caused by degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is a very slow progressing form. It has a wide range of onset from childhood to adult. Most patients remain ambulatory.
SIMILARITY: Belongs to the InsP3 receptor family. & Contains 5 MIR domains.
MISCELLANEOUS: Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium- binding protein which then inhibits the receptor.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB5882-200UL 04053252509131

Documentation

Anti-IP3 Receptor 1 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-IP3 Receptor 1 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-IP3 RECEPTOR 1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2159622 2159622
RABBIT ANTI-IP3 RECEPTOR 1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2435031 2435031
RABBIT ANTI-IP3 RECEPTOR 1 - 3220361 3220361
RABBIT ANTI-IP3 RECEPTOR 1 - 3286085 3286085
RABBIT ANTI-IP3 RECEPTOR 1 - 3695360 3695360
RABBIT ANTI-IP3 RECEPTOR 1 - 4030982 4030982
RABBIT ANTI-IP3 RECEPTOR 1 - 4128292 4128292
RABBIT ANTI-IP3 RECEPTOR 1 -2695234 2695234
RABBIT ANTI-IP3 RECEPTOR 1 -2736452 2736452
RABBIT ANTI-IP3 RECEPTOR 1 -2755393 2755393
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Data Sheet

Title
RABBIT ANTI-IP3 RECEPTOR 1 AFFINITY PURIFIED POLYCLONAL ANTIBODY