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ABF188-25UG Anti-IRF-1

Anti-IRF-1, Cat. No. ABF188, is a rabbit polyclonal lantibody that detects Interferon regulatory factor 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-IRF-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: IRF1 UniProt Number: P10914
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ABF188-25UG
25 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABF188-25UG
DescriptionAnti-IRF-1
Alternate Names
  • Interferon regulatory factor 1
  • IRF1
Background InformationInterferon regulatory factor 1 (UniProt: P10914; also known as IRF-1) is encoded by the IRF1 gene (Gene ID: 3659) in human. IRF-1 is a transcriptional regulator that is expressed constitutively in all cells types except early embryonal cells. It can exist as a monomer and as a homodimer. It displays remarkable functional diversity in the regulation of cellular responses, including the regulation of interferon (IFN) and IFN-inducible genes, host response to viral and bacterial infections. It also regulates many genes expressed during hematopoiesis, inflammation, immune responses, cell proliferation and differentiation, and growth arrest and apoptotic cell death following DNA damage. IRF-1 is reported to stimulate p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53. Its DNA-binging domains are located in the first 115 amino acids of N-terminal region and contains a series of five well-conserved tryptophan-rich repeats. IRF-1 can be phosphorylated by casein kinase 2, which positively regulates its activity. Mutations in IRF1 gene are reported to cause gastric cancer. (Ref.: Dou, L., et al. (2014). Hum. Immunol. 75(11); 1110-1114).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-IRF-1, Cat. No. ABF188, is a rabbit polyclonal lantibody that detects Interferon regulatory factor 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected IRF-1 in Jurkat cell lysate.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected IRF-1 in human stomach cancer and human small intestine tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal half of human Interferon Regulatory Factor 1.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Interferon Regulaotry Factor 1 in human cells. It targets an epitope within 14 amino acids from the N-terminal half.
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat, Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • IRF1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~48 kDa observed; 36.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IRF-1 in PC12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABF188-25UG 04054839448089