Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

ABF202 Anti-IRF7 Antibody

This Anti-IRF7 Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunofluorescence for the detection of IRF7.

Anti-IRF7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: IRF7 UniProt Number: Q92985
View Products on Sigmaaldrich.com
ABF202
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHC, IFRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABF202
DescriptionAnti-IRF7 Antibody
Alternate Names
  • Interferon regulatory factor 7
  • IRF7
Background InformationInterferon regulatory factor 7 (IRF7) is a member of the IRF family and has 1 IRF tryptophan pentad repeat DNA-binding domain. IRF7 is key transcriptional regulator of type I interferon (IFN)-dependent immune responses and regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. IRF7 plays an important role in the innate immune response against DNA and RNA viruses and has been shown to activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. IRF7 exists in an inactive form in the cytoplasm of uninfected cells and following viral infection it becomes phosphorylated by IKBKE and TBK1 kinases. This leads to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Studies show that IRF7 induces PSMB9/LMP2 expression, either directly or through induction of IRF1 and may play a role in regulating adaptive immune responses. Other evidence shows that IRF7 binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Additionally, IRF7 is implied to activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. IRF7 is expressed predominantly in spleen, thymus and peripheral blood leukocytes.
References
Product Information
FormatAffinity Purified
Control
  • HEK293 whole cell lysate
PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-IRF7 Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunofluorescence for the detection of IRF7.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: 5 µg/mL from a representative lot detected IRF7 in mouse spleen tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected IRF7 in mouse spleen cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human IRF7.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the C-terminus of IRF7.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Gene Symbol
  • IRF7
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~51 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 whole cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected IRF7 in HEK293 whole cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABF202 04053252923425