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05-784R Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal

Detect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: Insulin/Energy Signaling Gene Symbol: IRS1, IRS-1, HIRS-1 UniProt Number: P35570
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05-784R
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, R, HWB, IPRbPurifiedMonoclonal Antibody
Description
Catalogue Number05-784R
DescriptionAnti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal
Alternate Names
  • insulin receptor substrate 1
Background InformationIRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. It is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer.
References
Product Information
FormatPurified
Control
  • 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates
PresentationPurified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% glycerol.
Quality LevelMQ100
Applications
ApplicationDetect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blot (SNAP ID) Analysis: 0.1 µg/mL from a previous lot detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6 and MCF7 cell lysates.

Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.
Biological Information
ImmunogenKLH- conjugated - linear peptide corresponding to the C-terminus of rat IRS1.
EpitopeC-terminus
Clone58-10C-31
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityAntibody recognizes the C-terminus of IRS1.
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
  • Human
Species Reactivity NoteReacts with mouse, rat and human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IRS1
  • IRS-1
  • HIRS-1
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. Ref.3

SUBUNIT STRUCTURE: Interacts with SOCS7 By similarity. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1.

PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor, and Ser-789 phosphorylation is increased in the liver of insulin-resistant rats.

Phosphorylation of Tyr-895 is required for GRB2-binding.

SEQUENCE SIMILARITIES: Contains 1 IRS-type PTB domain.

Contains 1 PH domain.
Molecular Weight ~ 160 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in 3T3/A31, 3T3/L1, L6 or MCF7 cell lysates.

Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-784R 04053252373909

Documentation

Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal SDS

Title

Safety Data Sheet (SDS) 

Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-IRS1, clone 58-10C-31 2475663
Anti-IRS1, clone 58-10C-31 - 2459055 2459055
Anti-IRS1, clone 58-10C-31 - 2009444 2009444
Anti-IRS1, clone 58-10C-31 - 2150090 2150090
Anti-IRS1, clone 58-10C-31 - 2207201 2207201
Anti-IRS1, clone 58-10C-31 - 2299140 2299140
Anti-IRS1, clone 58-10C-31 - 2302240 2302240
Anti-IRS1, clone 58-10C-31 - 2377415 2377415
Anti-IRS1, clone 58-10C-31 - 2563501 2563501
Anti-IRS1, clone 58-10C-31 - NG1861488 NG1861488
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Technical Info

Title
Protein Blotting Handbook: 6th Edition