Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABD131 Anti-ISL1 Antibody, clone 1H9

Detect ISL1 using this mouse monoclonal antibody, Anti-ISL1 Antibody, clone 1H9 validated for use in western blotting, Immunofluorescence & IHC.

Anti-ISL1 Antibody, clone 1H9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: ISL1 UniProt Number: P61371
View Products on Sigmaaldrich.com
MABD131
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IF, IHCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABD131
DescriptionAnti-ISL1 Antibody, clone 1H9
Alternate Names
  • Insulin gene enhancer protein ISL-1
  • Islet-1
Background InformationIslet-1 (ISL-1) is a transcription factor that plays an important role in the embryogenesis of pancreatic islets of Langerhans. It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. In mouse embryos, a deficiency of this gene fail to undergo neural tube motor neuron differentiation. More recently it has been identified as a marker for a cardiac progenitor cell lineage that is capable of differentiating into all 3 major heart cell types: cardiomyocytes, smooth muscle and endothelial cell lineages.
References
Product Information
FormatAscites
Control
  • Full-length ISL1-hIgGFc transfected HEK293 cell lysate
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect ISL1 using this mouse monoclonal antibody, Anti-ISL1 Antibody, clone 1H9 validated for use in western blotting, Immunofluorescence & IHC.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunohistochemistry
Application NotesImmunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected ISL1 in full-length ISL1-hIgGFc transfected HEK293 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ISL1 in paraffin-embedded human lung cancer and cervical carcinoma tissues.

Optimal working dilutions must be determined by end user.

Biological Information
ImmunogenPurified recombinant fragment of human ISL1 expressed in E. Coli. corresponding to human ISL1.
Clone1H9
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • ISL1
Purification MethodUnpurified
UniProt Number
Molecular Weight~93 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in full-length ISL1-hIgGFc transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ISL1 in full-length ISL1-hIgGFc transfected HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABD131 04053252916458