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MAB2059-C Anti-Integrin B4 Antibody, clone ASC-8, Azide & Ascites Free

This Anti-Integrin B4 Antibody, clone ASC-8, Azide & Ascites Free is validated for use in flow cytometry, inhibition, IHC & IP for the detection of Integrin B4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: ITGB4 UniProt Number: P16144
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MAB2059-C
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, Inhibition, IHC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2059-C
DescriptionAnti-Integrin B4 Antibody, clone ASC-8, Azide & Ascites Free
Alternate Names
  • Integrin beta-4
  • Integrin B4
  • Integrin Beta4
  • GP150
  • CD104
Background InformationIntegrin proteins couple the ECM outside of a cell to the cytoskeleton inside of the cell. Which ECM protein binds and what signals are conveyed depend upon the particular makeup of the integrin pair being expressed. Functional integrin receptors are made up of two protein chains linked by disulfide bonds, an alpha chain and a beta chain. Integrin beta 4, also known as CD104, or GP160 is one of eight different beta chains, however beta 4 integrin is part of only one principal integrin receptor, integrin alpha6beta4. This receptor is expressed in endothelium, epithelium, and perineurium tissues of several organs as well as fibroblasts and Schwann cells but not in blood or lymphatic cells. Integrin alpha6beta4 is the laminin receptor and is critical for cellular polarity and motility.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-Integrin B4 Antibody, clone ASC-8, Azide & Ascites Free is validated for use in flow cytometry, inhibition, IHC & IP for the detection of Integrin B4.
Key Applications
  • Flow Cytometry
  • Inhibition
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A representative lot detected Integrin B4 in acetone-fixed frozen human epithelial, tongue, foreskin, and ovary tissues. (Note: Not for use on formalin-fixed tissues.)

Inhibition Assay Analysis: A representative lot inhibits SCC9 cell adhesion to human and EHS laminin and laminin GD-2 peptides, but not to type IV collagen or fibronectin. It does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.

Flow Cytometry Analysis: A representative lot detected Integrin B4 in SCC9, SKOV-3, HUVEC cells. It also detected Integri B4 in RKO and K562 cells transfected for CD104 expression. It does not stain Integrin B4 in monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin B4 from surface-biotinylated SCC9 cells. Immunoprecitated Integrin B4 was then observed at 66 kDa, 76 kDa, 120 kDa, 135 kDa and 200kDa (reduced), or 66 kDa, 110 kDa, 125 kDa, 130 kDa and 190kDa (non-reduced) in PAGE.
Biological Information
ImmunogenSCC9 whole cells expressing Integrin B4.
CloneASC-8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ITGB4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight202 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in A431 cells.

Flow Cytometry Analysis: 0.25 µg of this antibody detected Integrin B4 in 1X10E6 A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB2059-C 04053252965296

Documentation

Anti-Integrin B4 Antibody, clone ASC-8, Azide & Ascites Free Certificates of Analysis

TitleLot Number
Anti-Integrin B4, clone ASC-8, Azide & Ascites Free - Q2395223 Q2395223
Anti-Integrin B4, clone ASC-8, Azide & Ascites Free - 2892497 2892497
Anti-Integrin B4, clone ASC-8, Azide & Ascites Free - 3163557 3163557
Anti-Integrin B4, clone ASC-8, Azide & Ascites Free - 3211923 3211923
Anti-Integrin B4, clone ASC-8, Azide & Ascites Free - 3330666 3330666
Anti-Integrin B4, clone ASC-8, Azide & Ascites Free - 3729993 3729993
Anti-Integrin B4, clone ASC-8, Azide & Ascites Free - 3953664 3953664
Anti-Integrin B4, clone ASC-8, Azide & Ascites Free - 4010928 4010928
Anti-Integrin B4, clone ASC-8, Azide & Ascites Free -2673194 2673194
Anti-Integrin B4, clone ASC-8, Azide & Ascites Free -2723665 2723665