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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABC309 Sigma-AldrichAnti-KIF1B Antibody, clone 10F3.1

Anti-KIF1B Antibody, clone 10F3.1 is an antibody against KIF1B for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-KIF1B Antibody, clone 10F3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: KIF1B, KIAA0591, KIAA1448 UniProt Number: O60333

Replacement Information

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, IH(P)	M	Culture Supernatant	Monoclonal Antibody

Description
Catalogue Number	MABC309
Description	Anti-KIF1B Antibody, clone 10F3.1
Alternate Names	KIF1B Kinesin-like protein KIF1B Klp
Background Information	Kinesin-like protein KIF1B (UniProt O60333; also known as Kinesin superfamily protein KIF1B, Klp) is encoded by the KIF1B (also known as KLP, CMT2, CMT2A, CMT2A1, HMSNII, KIAA0591, KIAA1448, NBLST1) gene (Gene ID 23095) in human. KIF1B is a member of the kinesin 3 family of proteins. The KIF1B gene encodes four alternatively spliced isoforms, among which isoform 2 and isoform 3 are also known as the alpha and beta isoforms. The alpha and beta isoforms share a common N-terminal motor domain but contain different C-terminal cargo domains. The alpha and beta isoforms are motor proteins implicated in anterograde transport of mitochondria and synaptic vesicle precursors, respectively. In addition, the beta isoform is required for developmental apoptosis induction by nerve growth factor (NGF) deprivation among neural crest progenitors during sympathetic neuron development. The beta isoform is reported to interact with RNA helicase A (DHX9), resulting in DHX9 nuclear accumulation and upregulated transcription of the proapoptotic XIAP-associated factor 1 (XAF1). KIF1B gene is located in a chromosomal region frequently deleted in neural crest–derived tumors, including neuroblastomas with loss of KIF1Bβ expression and impaired DHX9 nuclear localization.

References

Product Information
Format	Culture Supernatant
Presentation	Mouse monoclonal IgG2aκ tissue culture supernatant without preservatives.
Quality Level	MQ100

Applications
Application	Anti-KIF1B Antibody, clone 10F3.1 is an antibody against KIF1B for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected KIF1B in human skeletal muscle, mouse brain, mouse kidney, rat skeletal muscle, and rat testis tissue.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the C-terminal region of human KIF1B.
Epitope	C-terminal region
Clone	10F3.1
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Expected to react with spliced isoforms 1 and 2 (isoform Beta), but not isoform 3 (isoform Alpha). Reactivity toward isoform 4 is highly likely, but not yet confirmed.
Isotype	IgG2aκ
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_055889
Gene Symbol	KIF1B KIAA0591 KIAA1448
Purification Method	Unpurified
UniProt Number	O60333
Molecular Weight	~204 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected KIF1B in 10 µg of SH-SY5Y cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 μL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
MABC309	04055977299984

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