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MABT1324-25UG Anti-Keratan sulfate Antibody, clone 373E1

Anti-Keratan sulfate, clone 373E1, Cat. No. MABT1324, is a mouse monoclonal antibody that detects keratan Sulfate and is tested for use in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation and Western Blotting.

MABT1324-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1324-25UG
DescriptionAnti-Keratan sulfate Antibody, clone 373E1
Alternate Names
  • KS
  • Keratosulfate
Background InformationKeratan sulfate (also known as keratosulfate) is a widely distributed glycosaminoglycan in tensional and weight-bearing connective tissues, epithelial tissues, and the central and peripheral nervous system. It is attached to a number of proteoglycan and glycoprotein core proteins. Three types of keratan sulfate have been described: KS I, KS II, and KS III depending on their distribution pattern. Keratan sulfate is the only glycosaminoglycan (GAG) that does not contain uronic acid; therefore, it is not cleavable by the eliminative enzymatic cleavage. The biosynthesis of keratan sulfate occurs in two stages. In the first stage, the region binding the core protein with GAG is created followed by the elongation of the chain and its modification. The elongation of keratan sulfate chains of all types occurs by alternate attaching of Gal and GlcNAc residues, catalyzed by β-1,4-galactotransferase and β-1,3-N-acetyloglucosaminotransferase, respectively. Sulfation of keratan sulfate is carried out by at least two sulfotransferases. Gal 6-O-sulfotransferase transfers sulfate to position 6 of Gal residues located in the internal region of keratan sulfate and GlcNAc 6-O-sulfotransferase transfers sulfate to position 6 of GalNAc residues located at the nonreducing terminal of keratan sulfate. Clone 373E1 is a monoclonal antibody that detects a unique sialylation/ conformational-dependent KS epitope that detects a novel glycoprotein complex produced by papillary thyroid carcinoma cells. (Ref.: Magro, G., et al. (2003) Am. J. Pathol. 163(1); 183-196; Caterson, B., et al. (1982). J. Invest. Dermatol. 79 (suppl 1); 45-50; Coleman, PJ., et al. (1998). J. Physiol. 509(3); 695-710).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-Keratan sulfate, clone 373E1, Cat. No. MABT1324, is a mouse monoclonal antibody that detects keratan Sulfate and is tested for use in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • ELISA
  • Western Blotting
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected Keratan Sulfate in Western Blotting application (Magro, G., et al. (2003). Am J Pathol. 163(1):183-96).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Keratan Sulfate in Immunoprecipitation application (Magro, G., et al. (2003). Am J Pathol. 163(1):183-96).

Immunohistochemistry Applications: A representative lot detected Keratan Sulfate in Immunohistochemistry application (Magro, G., et al. (2003). Am J Pathol. 163(1):183-96).

ELISA Analysis: A representative lot detected Keratan Sulfate in ELISA application (Magro, G., et al. (2003). Am J Pathol. 163(1):183-96).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenPurified proteoglycans from embryonic chicken.
Clone373E1
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 373E1 is a mouse monoclonal antibody that detects Keratan sulfate in cartilage tissue.
IsotypeIgMκ
Species Reactivity
  • Human
  • Chicken
Species Reactivity NoteChicken, Human. Predicted to react with Vertebrates.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodLigatrap
UniProt Number
Molecular WeightN/A
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in Human cartilage tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Keratan Sulfate in human cartilage tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT1324-25UG 04065270453556