Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABT1456-25UL Anti-LAMC2 Antibody, clone F2H4

This mouse monoclonal Anti-LAMC2, clone F2H4, Cat. No. MABT1456, is a mouse monoclonal antibody that detects Laminin subunit gamma-2 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

MABT1456-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1456-25UL
DescriptionAnti-LAMC2 Antibody, clone F2H4
Alternate Names
  • Laminin subunit gamma-2
  • Cell-scattering factor 140 kDa subunit
  • CSF 140 kDa subunit
  • Epiligrin subunit gamma
  • Kalinin subunit gamma
  • Kalinin/nicein/epiligrin 100 kDa subunit
  • Ladsin 140 kDa subunit
  • Laminin B2t chain
  • Laminin-5 subunit gamma
  • Large adhesive scatter factor 140 kDa subunit
  • Nicein subunit gamma
Background InformationLaminin subunit gamma-2 (UniProt: Q13753; also known as Cell-scattering factor 140 kDa subunit, CSF 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as LAMB2T, LAMNB2) gene (Gene ID: 3918) in human. Laminin is a major basement membrane glycoprotein consisting of a, b, and g chains that are bound to each other by disulfide bonds. Over 15 different isoforms have been reported in the laminin family that are expressed in a tissue-specific manner and play different role in each tissue. g 2, a subunit of Laminin-5 (also known as Laminin-332) is reported to be frequently over-expressed as a monomer or as the b3- g 2 heterodimer in invasive cancers and is regarded as an invasion marker. It has 8 EGF-like domains. The cell adhesion and motility activities of Laminin-5 are reported to be regulated by the proteolytic cleavage of the short arm of Laminin g 2 chain by BMP-1 or MT1-MMP. This cleavage releases a 45 kDa N-terminal proteolytic fragment (g 2pf). The short arm and g 2pf can be further cleaved by serine proteases and by matrix metalloproteinases resulting in the release of small N-terminal fragments. g 2pf‐like fragments have been reported to be present in conditioned media of cultured cancer cells and in sera from human cancer subjects. Clone F2H4 targets an epitope within NE1 of domain V of Laminin g 2 and shows reactivity with Laminin g 2, g 2pf and N-terminal fragments. (Ref.: Sato, H., et al. (2014). Cancer Sci. 105(2); 168-175; Miyazaki, K., et al. (2016). Cancer Sci. 107(12); 1909-1918).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationThis mouse monoclonal Anti-LAMC2, clone F2H4, Cat. No. MABT1456, is a mouse monoclonal antibody that detects Laminin subunit gamma-2 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • ELISA
  • Western Blotting
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected LAMC2 in Purified Lmg2pf and purified Lmg2 full length protein (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Immunohistochemistry Applications: A representative lot detected LAMC2 in Immunohistochemistry applications in Squamous cell carcinoma of the uterus and lung carcinoma tissues (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

ELISA Analysis: 5 µg/mL from a representative lot detected LAMC2 in Lm-gamma2pf and Lm-gamma2 in Lm332 condition medium from Lm332-expressing HEK293 cells (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged full-length human Laminin subunit gamma-2 (LAMC2).
EpitopeUnknown
CloneF2H4
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone F2H4 is a mouse monoclonal antibody that specifically detects human Laminin subunit gamma-2 (LAMC2). It targets an epitope within the NE1 repeat of domain V.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LAMC2
  • LAMB2T
  • LAMNB2
Purification MethodProtein G purified
UniProt Number
Molecular Weight130.98 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected LAMC2 in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT1456-25UL 04065265398060