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ABC929 Anti-LC3-I/II Antibody

Anti-LC3-I/II, Cat. No. ABC929, is a rabbit polyclonal antibody that detects LC3B-I/II and is tested for use in Immunocytochemistry and Western Blotting.

Anti-LC3-I/II Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: LC3, LC3A, MAP1A, MAP1BLC3A, MAP1LC3A, LC3I, LC3II UniProt Number: Q9H492
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ABC929
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC929
ReplacesMABC177; MABC176
DescriptionAnti-LC3-I/II Antibody
Alternate Names
  • Microtubule-associated proteins 1A/1B light chain 3A
  • Autophagy-related protein LC3 A
  • Autophagy-related ubiquitin-like modifier LC3 A
  • MAP1 light chain 3-like protein 1
  • MAP1A/MAP1B light chain 3 A
  • Microtubule-associated protein 1 light chain 3 alpha
Background InformationMicrotubule-associated proteins 1A/1B light chain 3B (UniProt: Q9GZQ8; also known as Autophagy-related protein LC3B, Autophagy-related ubiquitin-like modifier LC3B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3B, Microtubule-associated protein 1 light chain 3 beta) is encoded by the MAP1LC3B (also known as MAP1ALC3) gene (Gene ID: 81631) in human. LC3 is ubiquitously distributed in all eukaryotes and is expressed as three splice variants known as LC3A, LC3B, and LC3C. They are structural proteins of autophagosomal membranes and are widely used as biomarkers of autophagy. They are ubiquitously distributed in all eukaryotes. They possess a ubiquitin-like structure despite differences in their respective amino acid sequences. The amino-terminus of LC3 is composed of an alpha-helix that varies among the 3 members. However, its C-terminus is conserved and consists of a ubiquitin core composed of beta strands with hydrophobic pockets. The ubiquitin core region is responsible for its interaction with Atg core machinery proteins. LC3 is cleaved by Atg4 between amino acids 120-121 to form LC3-I and expose its C-terminal glycine. LC3-I then conjugates to phosphatidylethanolamine (PE) by Atg7, Atg3, and the Atg12 complex to generate LC3-II, which is inserted into autophagosome membrane. LC3B is reported to be most abundant in heart, brain, skeletal muscle and testis and only a trivial expression is observed in the liver. Higher expression of LC3B in triple negative breast cancer is associated with poor outcomes in patients. It plays a role in mitophagy and regulates mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess reactive oxygen species production. C3B is synthesized with a propeptide (aa 121-125), which is cleaved off to produce the mature form. LC3B can undergo phosphorylation at threonine 12 by protein kinase A (PKA) and this phosphorylation inhibits its conjugation to phosphatidylethanolamine. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, YK and Lee JA (2016). BMB Rep. 49(8): 424-430).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-LC3-I/II, Cat. No. ABC929, is a rabbit polyclonal antibody that detects LC3B-I/II and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LC3-I/II in HeLa cells treated with 100 mM Chloroquine.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged full-length recombinant human Autophagy-related ubiquitin-like modifier LC 3B (LC3).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Autophagy-related ubiquitin-like modifier LC3B (LC3 I/II).
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Fish, Mouse, Porcine, Rat, Xenopus, and Zebra Fish based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • LC3
  • LC3A
  • MAP1A
  • MAP1BLC3A
  • MAP1LC3A
  • LC3I
  • LC3II
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~14/15 kDa observed; 14.69 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HeLa cells treated with 100 mM Chloroquine.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LC3-I/II in lysate from HeLa cells treated with 100 mM Chloroquine (3 h).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABC929 04053252986420