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ABE1367-100UG Anti-LCoR (Splice Variant)

Anti-LCoR, Cat. No. ABE1367, is a highly specific rabbit polyclonal antibody that targets a splice variant form of Ligand-dependent corepressor (LCoR) and has been tested in Immunocytochemistry and Western Blotting.

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ABE1367-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberABE1367-100UG
DescriptionAnti-LCoR (Splice Variant)
Alternate Names
  • Ligand-dependent corepressor
  • Mblk1-related protein 2
  • C10ORF12
  • LCoR (splice variant)
  • PALI1
Background InformationLigand-dependent corepressor (UniProt: Q96JN0; also known as LCoR, Mblk1-related protein 2) is encoded by the LCOR (also known as C10orf12, KIAA1795, MLR2) gene (Gene ID: 84458) in human. LCoR is a ubiquitously distributed protein in fetal and adult tissues and serves as a transcriptional co-repressor that is recruited to agonist-bound nuclear receptors through a single LxxLL motif at the N-terminal region. It may also act as a transcription activator that binds DNA elements with the sequence 5'-CCCTATCGATCGATCTCTACCT-3'. Three isoforms of LCoR have been described that are produced by alternative splicing. This polyclonal antibody is raised against a large isoform (isoform 3; a splice variant), which associates with the PRC2 complex through its C-terminal region. Its nuclear localization signal is reported to be in amino acids 339-345 and it reacts with nuclear receptors via amino acids 53-57. (Ref.: Conway, E., et al. (2018). Mol. Cell 70(3); 408-421).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-LCoR, Cat. No. ABE1367, is a highly specific rabbit polyclonal antibody that targets a splice variant form of Ligand-dependent corepressor (LCoR) and has been tested in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected LCoR in whole cell lysates from Pali1 KO and matched wild type mouse embryonic stem (Courtesy of Dr. Eric Conway, Dr. Emilia Jerman & Prof. Adrian P. Bracken at the Smurfit Institute of Genetics, Trinity College Dublin, Ireland).

Western Blotting Analysis: A representative lot detected LCoR in Western Blotting applications (Conway, E., et. al. (2018). Mol. Cell. 70(3):408-421).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human Ligand-dependent corepressor (LCoR), isoform 3.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects a splice variant of human and murine Ligand-dependent corepressor (LCoR). It targets an epitope with in the N-terminal half.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • LCOR
  • C10orf12
  • KIAA1795
  • MLR2
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~250 kDa observed; 170.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in NTERA2 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LCoR in NTERA2 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE1367-100UG 04054839757525

Documentation

Anti-LCoR (Splice Variant) Certificates of Analysis

TitleLot Number
Anti-LCoR (Splice Variant) - 3455243 3455243
Anti-LCoR (Splice Variant) - 3910139 3910139
Anti-LCoR (Splice Variant) - Q3207404 Q3207404

References

Reference overviewPub Med ID
A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities.
Conway, E; Jerman, E; Healy, E; Ito, S; Holoch, D; Oliviero, G; Deevy, O; Glancy, E; Fitzpatrick, DJ; Mucha, M; Watson, A; Rice, AM; Chammas, P; Huang, C; Pratt-Kelly, I; Koseki, Y; Nakayama, M; Ishikura, T; Streubel, G; Wynne, K; Hokamp, K; McLysaght, A; Ciferri, C; Di Croce, L; Cagney, G; Margueron, R; Koseki, H; Bracken, AP
Mol Cell  70  408-421.e8  2018

Show Abstract 		29628311 29628311