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05-939-I-100UL Anti-LSD1/BHC110 Antibody, clone Clone AP06.20

Anti-LSD1/BHC110, Cat. No. 05-939-I, is a mouse monoclonal antibody that detects Lysine-specific histone demethylase 1A and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, and Western Blotting.

05-939-I-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number05-939-I-100UL
DescriptionAnti-LSD1/BHC110 Antibody, clone Clone AP06.20
Alternate Names
  • Lysine-specific histone demethylase 1A
  • BRAF35-HDAC complex protein BHC110
  • Flavin-containing amine oxidase domain-containing protein 2
Background InformationLysine-specific histone demethylase 1A (UniProt: O60341; also known as BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, LSD1) is encoded by the KD1MA (also known as AOF2, KDM1, KIAA0601, LSD1) gene (Gene ID: 23028) in human. LSD1 is a ubiquitously expressed histone demethylase that can demethylate both lysine 4 (H3K4me) and lysine 9 (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor. It is a component of a RCOR/GFI/KDM1A/HDAC complex that suppresses several genes implicated in multilineage blood cell development. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. It can also act as a corepressor by mediating demethylation of mono- and di-methylated H3K4me, a specific tag for epigenetic transcriptional activation. It has been reported that alone it is not able to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. LSD1 is also shown to act as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. LSD1 is also involved in demethylation of lysine 370 on p53/TP53 that blocks interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Two isoforms of LSD1 have been described that are produced by alternative splicing.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-LSD1/BHC110, Cat. No. 05-939-I, is a mouse monoclonal antibody that detects Lysine-specific histone demethylase 1A and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, and Western Blotting.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected LSD1/BHC110 in HEK293 cell nuclear extract.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected LSD1/BHC110 in Chromatin Immunoprecipitation applications (Brasacchio, D., et. al. (2009). Diabetes. 58(5):1229-36).

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected LSD1/BHC110 in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 116 amino acids from the N-terminal half of human Lysine-specific histone demethylase 1A (LSD1/BHC110).
EpitopeN-terminus
CloneAP06.20
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone AP06.20 is a mouse monoclonal antibody that detects Lysine-specific histone demethylase 1A (LSD1). It targets an epitope within the N-terminal half.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KD1MA
  • AOF2
  • KDM1
  • KIAA0601
  • LSD1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~110 kDa observed; 92.90 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Hela cell nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LSD1/BHC110 in HeLa cell nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-939-I-100UL 04061841878216

Documentation

Anti-LSD1/BHC110 Antibody, clone Clone AP06.20 SDS

Title

Safety Data Sheet (SDS) 

Anti-LSD1/BHC110 Antibody, clone Clone AP06.20 Certificates of Analysis

TitleLot Number
Anti-LSD1/BHC110, clone Clone AP06.20 - Q3518007 Q3518007

References

Reference overviewPub Med ID
Hyperglycemia induces a dynamic cooperativity of histone methylase and demethylase enzymes associated with gene-activating epigenetic marks that coexist on the lysine tail
Daniella Brasacchio 1 , Jun Okabe, Christos Tikellis, Aneta Balcerczyk, Prince George, Emma K Baker, Anna C Calkin, Michael Brownlee, Mark E Cooper, Assam El-Osta
Diabetes  58(5)  1229-36  2009

Show Abstract 		19208907 19208907