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ABS1621 Anti-Lcat Antibody

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ABS1621
100 µL  
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Overview

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABS1621
DescriptionAnti-Lcat Antibody
Alternate Names
  • Phosphatidylcholine-sterol acyltransferase
  • Lecithin-cholesterol acyltransferase
  • Phospholipid-cholesterol acyltransferase
  • Lcat
Background InformationPhosphatidylcholine-sterol acyltransferase (EC 2.3.1.43; UniProt P16301; also known as Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase, LCAT) is encoded by the Lcat (also known as AI046659; D8Wsu61e) gene (Gene ID 16816) in murine species. LCAT catalyzes the esterification of cholesterol and phosphatidylcholines (PCs) to cholesteryl esters and lysoPCs on the surface of high and low density lipoproteins (HDLs and LDLs). LCAT reaction is essential for the maturation of nascent HDL particles in plasma, and a genetic deficiency of LCAT results in the near absence of normal HDL particles and the presence of plasma very low density and low density lipoprotein particles of abnormal size and shape. LCAT is initially produced with a 24-aa N-terminal signal peptide, which is later cleaved to form the mature enzyme. LCAT is synthesized in the liver before it is secreted into the plasma.
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Lcat Antibody is validated for use in Western Blotting for the detection of Lcat .
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Lcat in FPLC-fractionated, TCA-precipitated plasma HDL from wild-type mice as well as mice with human apoM and/or A-I transgene (Liu, M., et al. (2014). J Biol Chem. 289(5)2801-2814).
Western Blotting Analysis: A representative lot detected Lcat in plasma from WT and endothelial lipase (EL) knockout mice (Ma, K., et al. (2003). Proc Natl Acad Sci USA. 100(5)2748-2753).
Western Blotting Analysis: A representative lot detected purified murine Lcat and Lcat in plasma samples from human A-I transgenic mice with or without scavenger receptor class B type I (SR-BI) knockout, purified mouse LCAT (Lee, J.Y., et al. (2007). J Lipid Res. 48(5)1052-1061).
Biological Information
ImmunogenRecombinant protein corresponding to mouse Lcat.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Mouse
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Lcat
Purification MethodUnpurified
UniProt Number
Molecular Weight~60 kDa observed. Target band size appears larger than the calculated molecular weight (49.7 kDa/preform & 49.0 kDa/mature) due to glycosylation. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse serum.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Lcat in ~2.3 µg of mouse serum.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalog Number GTIN
ABS1621 04055977175318