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07-1385 Anti-Lin28 Antibody

Anti-Lin28 Antibody detects level of Lin28 & has been published & validated for use in WB & IP.

Anti-Lin28 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Signaling Neuroscience, RNA Metabolism & Binding Proteins, RNA Binding Protein (RBP) Gene Symbol: LIN-28 , LIN28A UniProt Number: Q9H9Z2
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07-1385
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IPRbSerumPolyclonal Antibody
Description
Catalogue Number07-1385
DescriptionAnti-Lin28 Antibody
Alternate Names
  • RNA-binding protein LIN-28
Background InformationLin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.
References
Product Information
FormatSerum
PresentationRabbit Serum
Quality LevelMQ100
Applications
ApplicationAnti-Lin28 Antibody detects level of Lin28 & has been published & validated for use in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation:
Independent lab validated this antibody in immunoprecipitation. The antibody immunoprecipitated Lin28 from Tera-2 embryonic carcinoma and mouse ES cells using 2μL of antibody per reaction.
Biological Information
ImmunogenFull-length His-tagged Lin28 recombinant protein (human).
HostRabbit
SpecificityDetects Lin28
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman and Mouse. Other species have not been tested.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • LIN-28
  • LIN28A
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression By similarity.

SUBUNIT STRUCTURE: During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent By similarity.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus › nucleolus. Note= Nucleolar localization observed in 10 - 15% of the nuclei in differentiated myotubes By similarity.

SPECIFICITY: Expressed in embryonic stem cells (ES cells), placenta and testis. Ref.7 Ref.8 Ref.9

DEVELOPMENTAL STAGE: Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid. Ref.7 Ref.8 Ref.9

INDUCTION: Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Ref.6 Ref.10

DOMAIN: The CSD domain is required for function in muscle differentiation By similarity.

SIMILARITY: Belongs to the lin-28 family.

Contains 2 CCHC-type zinc fingers.

Contains 1 CSD (cold-shock) domain.
Molecular Weight25 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceWestern Blot Analysis:
This lot detected Lin28 at 1:1,000 dilution in Tera-2 embryonic carcinoma cell lysate resolved via SDS-PAGE and transferred to PVDF.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Store at -80°C for longer term storage. Avoid repeated freeze/thaws.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-1385 04053252281648

Documentation

Anti-Lin28 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Lin28 Antibody Certificates of Analysis

TitleLot Number
Anti-Lin28 3082653
Anti-Lin28 - 2443073 2443073
Anti-Lin28 - 2459568 2459568
Anti-Lin28 - 2035082 2035082
Anti-Lin28 - 4168644 4168644
Anti-Lin28 - NG1579727 NG1579727
Anti-Lin28 - NG1865042 NG1865042
Anti-Lin28 Polyclonal Antibody 2930117

References

Reference overviewPub Med ID
Selective blockade of microRNA processing by Lin28.
Viswanathan, Srinivas R, et al.
Science, 320: 97-100 (2008)  2008

18292307 18292307
Depletion of human micro-RNA miR-125b reveals that it is critical for the proliferation of differentiated cells but not for the down-regulation of putative targets during differentiation.
Lee, Yong Sun, et al.
J. Biol. Chem., 280: 16635-41 (2005)  2005

15722555 15722555
Micro-RNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells.
Wu, Ligang and Belasco, Joel G
Mol. Cell. Biol., 25: 9198-208 (2005)  2005

16227573 16227573

Brochure

Title
RNA-Binding Protein Immunoprecipitation