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MAB366 Anti-MAP1B Antibody, clone AA6

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MAB366
100 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, Ch, Ht, M, R, FeWB, IHCMAscitesMonoclonal Antibody
Description
Catalogue NumberMAB366
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MAP1B Antibody, clone AA6
Alternate Names
  • MAP5
References
Product Information
FormatAscites
PresentationAscites. Liquid containing 15mM sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MAP1B Antibody, clone AA6 detects level of MAP1B & has been published & validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunoblotting at 1:300-1:500.

Immunohistochemistry

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenRat brain microtubule-associated protein.
CloneAA6
HostMouse
SpecificityReacts specifically with MAP1B (MAP5) by immunoblot. Shows selective staining of dendritic trees throughout the brain. No cross-reactivity observed to other MAPs or tubulin.

Monoclonal Anti-MAP1b is immunospecific for MAP1b (also known as MAP5, MAPIX or MAP1.2; Riederer et al., 1986; Tucker et al., 1988) as determined by an immunoblot assay. Addition of antibody to microtubule proteins before polymerization results in abnormally short (but otherwise morphologically normal) microtubules.
IsotypeIgG1
Species Reactivity
  • Bovine
  • Chicken
  • Hamster
  • Mouse
  • Rat
  • Feline
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system.
Gene Symbol
  • MAP1B
  • DKFZp686F1345
  • FUTSCH
  • DKFZp686E1099
  • MAP5
  • FLJ38954
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P46821 # The function of brain MAPS is essentially unknown. Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B Binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules.
SIZE: 2468 amino acids; 270620 Da
SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. LC1 interacts with the amino- terminal region of MAP1B. Interacts with ANP32A (By similarity).
DOMAIN: SwissProt: P46821 Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules.
PTM: LC1 is generated from MAP1B by proteolytic processing. & Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the MAP1 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB366 04053252266676

Documentation

Anti-MAP1B Antibody, clone AA6 SDS

Title

Safety Data Sheet (SDS) 

Anti-MAP1B Antibody, clone AA6 Certificates of Analysis

TitleLot Number
MOUSE ANTI-MAP1B [MAP5] MONOCLONAL ANTIBODY - 2430371 2430371
MOUSE ANTI-MAP1B [MAP5] - 3195967 3195967
MOUSE ANTI-MAP1B [MAP5] - 3352408 3352408
MOUSE ANTI-MAP1B [MAP5] - 3755462 3755462
MOUSE ANTI-MAP1B [MAP5] -2502934 2502934
MOUSE ANTI-MAP1B [MAP5] -2551848 2551848
MOUSE ANTI-MAP1B [MAP5] -2603401 2603401
MOUSE ANTI-MAP1B [MAP5] MONOCLONAL ANTIBODY 3032745
MOUSE ANTI-MAP1B [MAP5] MONOCLONAL ANTIBODY 3061245
MOUSE ANTI-MAP1B [MAP5] MONOCLONAL ANTIBODY -2500385 2500385

References

Reference overviewSpeciesPub Med ID
Multiple functional domains and complexes of the two nonstructural proteins of human respiratory syncytial virus contribute to interferon suppression and cellular location.
Swedan, S; Andrews, J; Majumdar, T; Musiyenko, A; Barik, S
Journal of virology  85  10090-100  2011

Show Abstract
21795342 21795342
Genesis of neuronal and glial progenitors in the cerebellar cortex of peripuberal and adult rabbits.
Ponti, G; Peretto, P; Bonfanti, L
PloS one  3  e2366  2008

Show Abstract
Rabbit18523645 18523645
Proteins released from degenerating neurons are surrogate markers for acute brain damage.
Robert Siman, Tracy K McIntosh, Kristie M Soltesz, Zhaoming Chen, Robert W Neumar, Victoria L Roberts
Neurobiology of disease  16  311-20  2004

Show Abstract
15193288 15193288
Neuronal microtubule-associated proteins in the embryonic avian spinal cord.
Tucker, R P, et al.
J. Comp. Neurol., 271: 44-55 (1988)  1988

Show Abstract
3385011 3385011
MAP5: a novel brain microtubule-associated protein under strong developmental regulation.
Riederer, B, et al.
J. Neurocytol., 15: 763-75 (1986)  1986

Show Abstract
3819779 3819779