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MABC632 Anti-MAP3K5 Antibody, clone 2E4

Anti-MAP3K5 Antibody, clone 2E4 is a highly specific mouse monoclonal antibody, that targets MAP3K5 & has been tested in western blotting, IHC, ICC, Flow Cytometry & ELISA.

Anti-MAP3K5 Antibody, clone 2E4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: MAP3K5, ASK1, MAPKKK5, MEKK5 UniProt Number: Q99683
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MABC632
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, ICC, FC, ELISAMAscitesMonoclonal Antibody
Description
Catalogue NumberMABC632
DescriptionAnti-MAP3K5 Antibody, clone 2E4
Alternate Names
  • Mitogen-activated protein kinase kinase kinase 5
  • Apoptosis signal-regulating kinase 1
  • ASK-1
  • MAPK/ERK kinase kinase 5
  • MEK kinase 5
  • MEKK 5
Background InformationMitogen-activated protein kinase kinase kinase 5 (MAP3K5), also known as, MAPK/ERK kinase 5 (MEK Kinase 5; MEKK 5) or Apoptosis signal-regulating kinase 1 (ASK-1) protein, is a serine/threonine kinase that is part of the MAP kinase signal transduction pathway. The MAP kinase pathway is central to several signaling cascades the help determine everything from cell fate in differentiation to cell survival in apoptosis. MAP3K5 is critical for innate immune response against numerous pathogens and MAP3K5 also mediates cells responses to various cellular stresses such as oxidative stress, and responses mediated by inflammatory signaling agents such as TNF or LPS. Moreover, MAP3K5 has has been found to be involved in cancer, diabetes, cardiovascular and neurodegenerative diseases. MAP3K5 is membrane associated though not membrane bound directly. Its expression thus is primarily endoplasmic reticulum but can become strongly perinuclear under some conditions. Found in most tissues, it is abundant in heart and pancreas. It's activity is regulated via phosphorylation-dephosphorylation processes as well as though its interactions with a many other protein partners to form numerous multimolecular cell signaling complexes. EMD-Millipore’s Anti-MAP3K5 mouse monoclonal, clone 2E4, has been tested in western blotting against recombinant human MAP3K5 protein, and in paraffin-embedded immunohistochemistry with human cancer tissues, and in immunofluorescent immunocytochemistry, and flow cytometry on human HeLa cells in culture.
References
Product Information
FormatAscites
Control
  • HepG2 cell lysate
PresentationMouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MAP3K5 Antibody, clone 2E4 is a highly specific mouse monoclonal antibody, that targets MAP3K5 & has been tested in western blotting, IHC, ICC, Flow Cytometry & ELISA.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
  • Flow Cytometry
  • ELISA
Application NotesImmunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected MAP3K5 in human pancreas, heart, breast cancer, and prostate tissues.

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected MAP3K5 in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MAP3K5 in HeLa cells.

ELISA: A representative lot detected MAP3K5 in ELISA (Direct).

Optimal working dilutions must be determined by end user
Biological Information
ImmunogenPurified recombinant fragment of human MAP3K5 expressed in E. coli.
Clone2E4
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MAP3K5
  • ASK1
  • MAPKKK5
  • MEKK5
Purification MethodUnpurified
UniProt Number
Molecular Weight~155 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MAP3K5 in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC632 04053252910005