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ST1641 Anti-MAP4K4 Mouse mAb (4A5)

This Anti-MAP4K4 Mouse mAb (4A5) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of MAP4K4.

Anti-MAP4K4 Mouse mAb (4A5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4

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ST1641
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
ST1641-100UG 100 μg
Description
OverviewRecognizes the ~140 kDa MAP4K4 protein in K-562 cells and human breast cancer tissue.
Catalogue NumberST1641
Brand Family Calbiochem®
SynonymsAnti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
Application Data
Detection of human MAP4K4 by immunoblotitng. Sample: Whole cell lysate from K-562 cells. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human MAP4K4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlK-562 cells, Human breast cancer tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 611-710 human MAP4K4, expressed as a GST fusion protein
Clone4A5
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ST1641-100UG 04055977223774

Documentation

Anti-MAP4K4 Mouse mAb (4A5) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human MAP4K4 by immunoblotitng. Sample: Whole cell lysate from K-562 cells. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human MAP4K4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~140 kDa MAP4K4 protein.
BackgroundThe protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-α signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 611-710 human MAP4K4, expressed as a GST fusion protein
Clone4A5
IsotypeIgG₁
Specieshuman
Positive controlK-562 cells, Human breast cancer tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review