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AB9298 Anti-MARCKS Antibody, Complete sequence

Anti-MARCKS Antibody, Complete sequence is an antibody against MARCKS for use in WB.

Anti-MARCKS Antibody, Complete sequence MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Vesicular Trafficking, Neurodegenerative Diseases Gene Symbol: MARCKS, MACS, 80K-L, FLJ14368, PRKCSL, phosphomyristin, MRACKS, PKCSL, FLJ90045 UniProt Number: P29966
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AB9298
50 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB9298
ReplacesAB5427
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MARCKS Antibody, Complete sequence
References
Product Information
FormatPurified
Control
  • POSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze MARCKS content by immunoblot.
PresentationSemi-purified immunoglobulin. Liquid in 120 mM NaCl, 25 mM Tris-HCl, pH 7.3 containing 0.01% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MARCKS Antibody, Complete sequence is an antibody against MARCKS for use in WB.
Key Applications
  • Western Blotting
Application NotesImmunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 70-85 kDa (depending on species) MARCKS protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 8%. Overnight incubation with the antibody is recommended.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenFull length recombinant rat MARCKS.
EpitopeComplete sequence
HostRabbit
SpecificityRecognizes MARCKS.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Gene Symbol
  • MARCKS
  • MACS
  • 80K-L
  • FLJ14368
  • PRKCSL
  • phosphomyristin
  • MRACKS
  • PKCSL
  • FLJ90045
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29966 # MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
SIZE: 332 amino acids; 31555 Da
PTM: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
SIMILARITY: SwissProt: P29966 ## Belongs to the MARCKS family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB9298 04053252735424

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies