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MABT1534-25UG Anti-MARVELD2/Tricellulin Antibody, clone N54

MABT1534-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1534-25UG
DescriptionAnti-MARVELD2/Tricellulin Antibody, clone N54
Alternate Names
  • MARVEL domain-containing protein 2
Background InformationMARVEL domain-containing protein 2 (UniProt: Q3UZP0; also known as Tricellulin) is encoded by the Marveld2 (also known as Mrvldc2, Tric) gene (Gene ID: 218518) in murine species. Tricellulin is a multi-pass membrane protein that is predominantly detected in small intestine, lung, and kidney and lower levels are detected in liver, testis, and brain. It is a member of the tight junction-associated Marvel protein family, which consists of occludin, tricellulin, and Marveld3. Tricellulin contains four transmembrane domains, two extracellular loops and large amino- and carboxyl-terminal cytoplasmic domains. It plays a role in the formation of tricellular tight junctions and of epithelial barriers and regulates F-actin organization through Cdc42. It is shown to bind to the Cdc42 guanine-nucleotide-exchange factor (GEF) Tuba via its N-terminal cytoplasmic domain and activate Cdc42, thereby accelerating organization of F-actin fibers at tricellular contacts. Tricellulin-knockdown epithelial cells exhibit irregular polygonal shapes with curved cell borders and impaired organization of F-actin fibers around tricellular contacts during cell-cell junction formation. Tricellulin is reported to be essential for normal hearing via its role in the separation of the endolymphatic and perilymphatic spaces of the organ of Corti in the inner ear, and for normal survival of hair cells in the organ of Corti. Mutations in Marveld2 gene are reported to cause nonsyndromic familial deafness. (Ref.: Ikenouchi, J., et al. (2005). J. Cell Biol. 171(6): 939-945; Oda, Y., et al. (2014). J. Cell Sci. 127(19); 4201-4212).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-MARVELD2/Tricellulin, clone N54, Cat. No. MABT1534, is a rat monoclonal antibody that detects MARVEL domain-containing protein 2 and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:25 dilution from a representative lot detected MARVELD2/Tricellulin in mouse liver and kidney tissue sections.

Western Blotting Analysis: A representative lot detected MARVELD2/Tricellulin in Western Blotting applications (Oda, Y., et. al. (2014). J Cell Sci. 127(Pt 19):4201-12; Ikenouchi, J., et. al. (2005). J Cell Biol. 171(6):939-45).

Immunocytochemistry Analysis: A representative lot detected MARVELD2/Tricellulin in Immunocytochemistry applications (Kim, N.K., et. al. (2015). PLoS One. 10(2):e0116931).

Immunofluorescence Analysis: A representative lot detected MARVELD2/Tricellulin in Immunofluorescence applications (Ikenouchi, J., et. al. (2005). J Cell Biol. 171(6):939-45; Oda, Y., et. al. (2014). J Cell Sci. 127(Pt 19):4201-12; Yokouchi, M., et. al. (2016). Elife. 5. pii: e19593).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 150 amino acids from the N-terminal region of murine Tricellulin.
CloneN54
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone N54 is a rat monoclonal antibody that specifically detects murine Tricellulin. It targets an epitope within the N-terminal cytoplasmic domain.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Marveld2
  • Mrvldc2
  • Tric
Purification MethodProtein G purified
UniProt Number
Molecular Weight~64 kDa observed; 63.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in EpH4 cell lystate.

Western Blotting Analysis: 1 µg/mL of this antibody detected MARVELD2/Tricellulin in EpH4 cell lystate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT1534-25UG 04061841030461

Documentation

Anti-MARVELD2/Tricellulin Antibody, clone N54 SDS

Title

Safety Data Sheet (SDS) 

Anti-MARVELD2/Tricellulin Antibody, clone N54 Certificates of Analysis

TitleLot Number
Anti-MARVELD2/Tricellulin, clone N54 - 3272879 3272879

References

Reference overviewPub Med ID
Epidermal cell turnover across tight junctions based on Kelvin's tetrakaidecahedron cell shape.
Yokouchi, M; Atsugi, T; Logtestijn, MV; Tanaka, RJ; Kajimura, M; Suematsu, M; Furuse, M; Amagai, M; Kubo, A
Elife  5  2016

Show Abstract
27894419 27894419
Downsloping high-frequency hearing loss due to inner ear tricellular tight junction disruption by a novel ILDR1 mutation in the Ig-like domain.
Kim, NK; Higashi, T; Lee, KY; Kim, AR; Kitajiri, S; Kim, MY; Chang, MY; Kim, V; Oh, SH; Kim, D; Furuse, M; Park, WY; Choi, BY
PLoS One  10  e0116931  2015

Show Abstract
25668204 25668204
Tricellulin regulates junctional tension of epithelial cells at tricellular contacts through Cdc42.
Oda, Y; Otani, T; Ikenouchi, J; Furuse, M
J Cell Sci  127  4201-12  2014

Show Abstract
25097232 25097232
Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells.
Ikenouchi, J; Furuse, M; Furuse, K; Sasaki, H; Tsukita, S; Tsukita, S
J Cell Biol  171  939-45  2005

Show Abstract
16365161 16365161