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ABE1321-25UG Anti-MBD6

Anti-MBD6, Cat. No. ABE1321, is a highly specific rabbit polyclonal antibody that targets Methyl-CpG-binding domain protein 6 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-MBD6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: MBD6, KIAA1887 UniProt Number: Q96DN6
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ABE1321-25UG
25 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1321-25UG
DescriptionAnti-MBD6
Alternate Names
  • Methyl-CpG-binding domain protein 6
  • Methyl-CpG-binding protein MBD6
Background InformationMethyl-CpG-binding domain protein 6 (UniProt: Q96DN6; also known as Methyl-CpG-binding protein MBD6) is encoded by the MBD6 (also known as KIAA1887) gene (Gene ID: 114785) in human. The methyl-CpG-binding domain (MBD) proteins are primary candidates for the readout of DNA methylation. MBD proteins require a functional MBD domain and methyl-CpGs, although some MBD proteins can also bind unmethylated DNA. MBD proteins are involved in transcriptional repression by histone modification, nucleosome remodeling or chromatin organization. MBD6 is highly expressed in testes. Its MBD domain is localized in the N-terminal region (aa 11-81) and it also contains proline-rich domains. MBD6 binds to heterochromatin, but does not interact with either methylated or unmethylated DNA. MBD6 is reported to interact directly with the human polycomb deubiquitinase complex, PR-DUB, which catalyzes deubiquitination of H2AK119. Mutations in MBD6 that lead to R883W alteration are reported to cause autism. (Ref.: Cukier, HN., et al. (2012). Autism Res. 5(6); 385-397; Du, Q., et al. (2015). Epigenomics 7(6); 1051-1073).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MBD6, Cat. No. ABE1321, is a highly specific rabbit polyclonal antibody that targets Methyl-CpG-binding domain protein 6 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MBD6 in HeLa, A431, HEK293, and NIH3T3 cells.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MBD6 in human skin and human stomach tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Methyl-CpG-binding domain protein 6.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Methyl-CpG-binding domain protein 6 in human and murine cells. It targets an epitope within 16 amino acids from the N-terminal half.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MBD6
  • KIAA1887
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~110 kDa observed; 101.20 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected MBD6 in SH-SY5Y cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE1321-25UG 04054839448225