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MABF3048-25UL Anti-MERS-CoV Nucleoprotein Antibody, clone 46

Anti-MERS-CoV Nucleoprotein, clone 46, Cat. No. MABF3048, is a mouse monoclonal antibody that detects MERS-CoV Nucleoprotein and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.

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MABF3048-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABF3048-25UL
DescriptionAnti-MERS-CoV Nucleoprotein Antibody, clone 46
Alternate Names
  • Nucleocapsid protein
  • NC
  • Protein N
Background InformationNucleoprotein (UniProt: R9UM87; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 14254601) in Middle East respiratory syndrome-related coronavirus (MERS-CoV). The MERS-CoV is a positive-strand RNA virus of the betacoronavirus genus within the Coronaviridae family that causes severe respiratory syndrome in human. The mature MERS-CoV contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Nucleoprotein of MERS-CoV is a homooligomeric multifunctional protein that plays roles in genome encapsidation, RNA synthesis during replication, host translational shutoff, and innate immunity disruption. It packages the positive strand viral genome RNA into helical ribonucleocapsid and is involved in virion assembly through its interactions with the viral genome and membrane protein M. The nucleoprotein is reportedly organized into two folded domains known as the N-terminal domain (NTD) and the C-terminal domain (CTD) that are separated by a disordered region (LKR) containing a serine/ arginine stretch. Clone 46 displays specificity for MERS-CoV nucleoprotein and does not show cross-reactivity with nucleoprotein from other related viruses, including SARS coronavirus. (Ref.: Nguyen, THV., et al. (2019). Acta Crystallogr. D. Struct. Biol. 75(1); 8-15; Yamaoka, Y., et al. (2016). Front. Microbiol. 7; Article 509).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-MERS-CoV Nucleoprotein, clone 46, Cat. No. MABF3048, is a mouse monoclonal antibody that detects MERS-CoV Nucleoprotein and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • ELISA
  • Immunoprecipitation
Application NotesELISA Analysis: A representative lot detected MERS-CoV Nucleoprotein in ELISA applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Western Blotting Analysis: A representative lot detected MERS-CoV Nucleoprotein in Western Blotting applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Immunoprecipitation Analysis: A representative lot immunoprecipitated MERS-CoV Nucleoprotein in Immunoprecipitation applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 292 amino acids from the nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV).
EpitopeUnknown
Clone46
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 46 is a mouse monoclonal antibody that detects nucleoprotein from Middle East respiratory syndrome-related coronavirus (MERS-CoV).
IsotypeIgG1κ
Species Reactivity
  • Virus
Species Reactivity NoteMiddle East respiratory syndrome-related coronavirus (MERS-CoV).
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • N
Purification MethodProtein G purified
UniProt Number
Molecular Weight~45 kDa observed; 45.05 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant MERS-CoV nucleoprotein.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected recombinant MERS-CoV Nucleoprotein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF3048-25UL 04065265872775