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ABN1374-100UG Anti-MID1

Anti-MID1, Cat. No. ABN1374, is a highly specific rabbit polyclonal antibody that targets E3 ubiquitin-protein ligase Midline-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category: Neuroscience Gene Symbol: MID1, FXY, RNF59, TRIM18, XPRF UniProt Number: O15344
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ABN1374-100UG
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbPurifiedPolyclonal Antibody
Description
Catalogue NumberABN1374-100UG
DescriptionAnti-MID1
Alternate Names
  • E3 ubiquitin-protein ligase Midline-1
  • EC: 2.3.2.27
  • Midin
  • Putative transcription factor XPRF
  • RING finger protein 59
  • RING finger protein Midline-1
  • RING-type E3 ubiquitin transferase Midline-1
  • Tripartite motif-containing protein 18
Background InformationE3 ubiquitin-protein ligase Midline-1 (UniProt: O15344; also known as EC: 2.3.2.27, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, RING-type E3 ubiquitin transferase Midline-1, Tripartite motif-containing protein 18) is encoded by the MID1 (also known as FXY, RNF59, TRIM18, XPRF) gene (Gene ID: 4281) in human. Midlin is homodimeric protein with E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination that results in deprotection of the catalytic subunit of protein phosphatase PP2A and its subsequent degradation by polyubiquitination. It can also form heterodimer with MID2. It associates with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis. It is highly expressed in fetal kidney, followed by brain and lung. Lower levels are observed in fetal liver. In the adult, it is most abundant in heart, placenta, and brain. Midlin has multiple zinc binding sites and possesses three zinc fingers (one RING type and two B box-type). Mutations in MID1 gene are reported to cause Opitz GBBB syndrome 1 that is characterized by reduced affinity for microtubules, hypertelorism, and genital-urinary defects.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MID1, Cat. No. ABN1374, is a highly specific rabbit polyclonal antibody that targets E3 ubiquitin-protein ligase Midline-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MID1 in human placenta and human cerebral cortex tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human E3 ubiquitin-protein ligase Midline-1.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects E3 ubiquitin-protein ligase Midline-1 (MID1) in Human cells. It targets an epitope within 13 amino acids from the N-terminal region.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Rat, and Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MID1
  • FXY
  • RNF59
  • TRIM18
  • XPRF
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~75 kDa observed; 75.25 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected MID1 in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABN1374-100UG 04054839441080

Documentation