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MABC1105-100UG Anti-MORC3 Antibody, clone 17A9

Anti-MORC3, clone 17A9, Cat. No. MABC1105, is a mouse monoclonal antibody that detects MORC family CW-type zinc finger protein 3 and is tested for use in Immunocytochemistry and Western Blotting.

MABC1105-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABC1105-100UG
DescriptionAnti-MORC3 Antibody, clone 17A9
Alternate Names
  • MORC family CW-type zinc finger protein 3
  • Nuclear matrix protein 2
  • Zinc finger CW-type coiled-coil domain protein 3
Background InformationMORC family CW-type zinc finger protein 3 (UniProt: Q14149; also known as Nuclear matrix protein 2, Zinc finger CW-type coiled-coil domain protein 3) is encoded by the MORC3 (also known as KIAA0136, NXP2, ZCWCC3) gene (Gene ID: 23515) in human. MORC3 is a homodimeric protein of the MORC protein family characterized by conserved structures consisting of Gyrase H, Hsp90, and MutL (GHL)-ATPase domain at its N-terminus and a CW type zinc finger domain (aa 404-454). It is expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, and liver. MORC3 serves as a nuclear factor that forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism. It is also shown to be a target of herpes simplelx virus 1 (HSV-1) ICP0-mediated degradation. It is shown to be essential for fully efficient recruitment of PML, Sp100, hDaxx, and H2AX to sites associated with HSV-1 genomes entering the host cell nucleus. MORC3 recruits TP53 and SP100 to PML-NBs, hence, it is also involved in the regulation of TP53 activity. It regulates p53 activation via MORC3-ATPase activity. It can also induce p53-dependent premature senescence. (Ref.: Takahashi, K., et al. (2007). Mol Biol Cell. 18(5): 1701 1709; Sloan, E., et al. (2016). J. virology. 90(19); 8621-8633).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-MORC3, clone 17A9, Cat. No. MABC1105, is a mouse monoclonal antibody that detects MORC family CW-type zinc finger protein 3 and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A representative lot detected MORC3 in Immunocytochemistry applications (Takahashi, K., et. al. (2007). Mol Biol Cell. 18(5):1701-9).

Western Blotting Analysis: A 1:250 dilution from a representative lot detected MORC3 in PC12 cell lysates.
Biological Information
ImmunogenHis-tagged full-length recombinant human MORC family CW-type zinc finger protein 3 (MORC3).
Clone17A9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 17A9 is a mouse monoclonal antibody that specifically detects MORC family CW-type zinc finger protein 3 (MORC3). It targets an epitope with the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MORC3
  • KIAA0136
  • NXP2
  • ZCWCC3
Purification MethodProtein G purified
UniProt Number
Molecular Weight~ 160 and 230 kDa observed; 107.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected MORC3 in NIH/3T3 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC1105-100UG 04054839986741

Documentation

Anti-MORC3 Antibody, clone 17A9 Certificates of Analysis

TitleLot Number
Anti-MORC3, clone 17A9 - Q3222082 Q3222082

References

Reference overviewPub Med ID
Dynamic regulation of p53 subnuclear localization and senescence by MORC3.
Takahashi, K; Yoshida, N; Murakami, N; Kawata, K; Ishizaki, H; Tanaka-Okamoto, M; Miyoshi, J; Zinn, AR; Shime, H; Inoue, N
Mol Biol Cell  18  1701-9  2007

Show Abstract Full Text Article		17332504 17332504