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NA27 Anti-MSH2 (Ab-2) Mouse mAb (FE11)

This Anti-MSH2 (Ab-2) Mouse mAb (FE11) is validated for use in Frozen Sections Immunoblotting Immunoprecipitation Paraffin Sections for the detection of MSH2 (Ab-2).

Anti-MSH2 (Ab-2) Mouse mAb (FE11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Mismatch Repair Protein 2

NA27
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
NA27-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and in colon tissue.
Catalogue NumberNA27
Brand Family Calbiochem®
SynonymsAnti-Mismatch Repair Protein 2
References
ReferencesBronner, C.E., et al. 1994. Nature 368, 258.
Papadopoulos, N., et al. 1994. Science 263, 1625.
Peltomäki, P.T. 1994. Annals Med. 26, 215.
Fishel, R., et al. 1993. Cell 75, 1027.
Leach, F.S., et al. 1993. Cell 75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative controlLoVo cells
Positive controlHCT116 or SW480 cells or colon tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting, Frozen Sections Leach, F.S., et al. 1996. Cancer Res. 56, 235. Paraffin Sections Thibodeau, S.N., et al. 1996. Cancer Res. 56, 4836.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (2 µg/ml, see application references)
Immunoblotting (0.2 µg/ml, see application references)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2 µg/ml, heat or proteinase K pre-treatment required, see application references)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena C-terminal fragment of human MSH2
ImmunogenHuman
CloneFE11
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
NA27-100UG 07790788053857

Documentation

Anti-MSH2 (Ab-2) Mouse mAb (FE11) Certificates of Analysis

TitleLot Number
NA27

References

Reference overview
Bronner, C.E., et al. 1994. Nature 368, 258.
Papadopoulos, N., et al. 1994. Science 263, 1625.
Peltomäki, P.T. 1994. Annals Med. 26, 215.
Fishel, R., et al. 1993. Cell 75, 1027.
Leach, F.S., et al. 1993. Cell 75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 RFH
SynonymsAnti-Mismatch Repair Protein 2
ApplicationFrozen Sections (2 µg/ml, see application references)
Immunoblotting (0.2 µg/ml, see application references)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2 µg/ml, heat or proteinase K pre-treatment required, see application references)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.
BackgroundThe MSH2 gene is one of at least 4 genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma, while germline MSH2 mutations are responsible for ~50% of inherited, non-polyposis colorectal carcinoma (HNPCC). MSH2 encodes a protein of 934 amino acids.
HostMouse
Immunogen speciesHuman
Immunogena C-terminal fragment of human MSH2
CloneFE11
IsotypeIgG₁
Specieshuman, mouse
Positive controlHCT116 or SW480 cells or colon tissue
Negative controlLoVo cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesBronner, C.E., et al. 1994. Nature 368, 258.
Papadopoulos, N., et al. 1994. Science 263, 1625.
Peltomäki, P.T. 1994. Annals Med. 26, 215.
Fishel, R., et al. 1993. Cell 75, 1027.
Leach, F.S., et al. 1993. Cell 75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
Application referencesImmunoblotting, Frozen Sections Leach, F.S., et al. 1996. Cancer Res. 56, 235. Paraffin Sections Thibodeau, S.N., et al. 1996. Cancer Res. 56, 4836.