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MABT495 Anti-MYL3, clone 7C1 Antibody

Detect MYL3 using this Anti-MYL3, clone 7C1 Antibody validated for use in western blotting & IHC.

Anti-MYL3, clone 7C1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: MYL3 UniProt Number: P08590
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MABT495
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWB, IHCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABT495
DescriptionAnti-MYL3, clone 7C1 Antibody
Alternate Names
  • Myosin light chain 3
  • Cardiac myosin light chain 1
  • CMLC1
  • Myosin light chain 1
  • slow-twitch muscle B/ventricular isoform
  • MLC1SB
  • Ventricular/slow twitch myosin alkali light chain
Background InformationMyosin light chain 3 (MYL3), also known as Cardiac myosin light chain 1 (CMLC1) or Myosin light chain 1 slow-twitch muscle B/ventricular isoform (MLC1SB) or Ventricular/slow twitch myosin alkali light chain (VLC1) functions as a regulatory light chain of myosin. Myosins are a large superfamily of motor proteins that move along actin filaments, while hydrolyzing ATP. Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains and four light chains. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Human myosin light chain has clinical application as a cardiac marker. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect MYL3 using this Anti-MYL3, clone 7C1 Antibody validated for use in western blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected MYL3 in human skeletal muscle and cardiac muscle tissues.
Biological Information
ImmunogenPurified recombinant fragment of human MYL3 expressed in E. Coli
Clone7C1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1
Species Reactivity
  • Rat
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MYL3
Purification MethodUnpurified
UniProt Number
Molecular Weight~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat fetal heart tissue lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected MYL3 in rat fetal heart tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT495 04053252955495