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07-672-I Anti-MYPT1 Antibody

Detect MYPT1 using this rabbit polyclonal antibody, Anti-MYPT1 Antibody validated for use in western blotting & IP.

Anti-MYPT1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Metabolic Hormones & Receptors Gene Symbol: PPP1R12A, MBS, MYPT1 UniProt Number: Q90623
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07-672-I
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-672-I
Replaces04-386
DescriptionAnti-MYPT1 Antibody
Alternate Names
  • Protein phosphatase 1 regulatory subunit 12A
  • 130 kDa myosin-binding subunit of smooth muscle myosin phosphatase
  • Myosin phosphatase-targeting subunit 1
  • Myosin phosphatase target subunit 1
  • PP1M subunit M110
  • Protein phosphatase myosin-binding subunit
Background InformationProtein phosphatase 1 regulatory subunit 12A (UniProt: Q90623; also known as 130 kDa myosin-binding subunit of smooth muscle myosin phosphatase, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, PP1M subunit M110, Protein phosphatase myosin-binding subunit, MYPT1) is encoded by the PPP1R12A (also known as MBS, MYPT1) gene (Gene ID: 396020) in human. MYPT1 is one of the subunits and an integral component of the Myosin phosphatase. It is detected in brain, lung, aorta, heart, gizzard, stomach, oviduct, spleen, kidney, and small intestine. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity. It is phosphorylated by Rho-associated kinases on serine and threonine residues. Phosphorylation at Threonine 695 is shown to inhibit myosin phosphatase activity and phosphorylation at Threonine 850 is reported to abolish myosin binding. Two isoforms of MYPT1 have been reported that are produced by alternative splicing.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect MYPT1 using this rabbit polyclonal antibody, Anti-MYPT1 Antibody validated for use in western blotting & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: 0.5µg from a representative lot immunoprecipitated MYPT1 from 0.5mg of HeLa cell lysate.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminus of chicken MYPT1.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects human Protein phosphatase 1 regulatory subunit 12A. It targets an epitope within 291 amino acids from the C-terminal region.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Chicken based on immunogen design.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PPP1R12A
  • MBS
  • MYPT1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~120/130 kDa observed; 111.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MYPT1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-672-I 04053252937378