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ABS1495 Anti-MYPT1 Antibody

Anti-MYPT1 Antibody is an antibody against MYPT1 for use in Western Blotting.

Anti-MYPT1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: PPP1R12A, MBS, MYPT1 UniProt Number: Q90623
ABS1495
200 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1495
DescriptionAnti-MYPT1 Antibody
Alternate Names
  • Protein phosphatase 1 regulatory subunit 12A
  • MYPT1
  • 130 kDa myosin-binding subunit of smooth muscle myosin phosphatase
  • Myosin phosphatase-targeting subunit 1
  • Myosin phosphatase target subunit 1
  • PP1M subunit M110
  • Protein phosphatase myosin-binding subunit
Background InformationChicken Protein phosphatase 1 regulatory subunit 12A (also known as PP1M subunit M110, Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, Smooth muscle myosin phosphatase myosin-binding subunit, Protein phosphatase 1, regulatory (inhibitor) subunit 12A, 130 kDa myosin-binding subunit of smooth muscle myosin phophatase) is encoded by the Gallus gallus PP1R12A (also known as MBS, MBSP, MYPT1) gene (Entrez Gene ID 396020). MYTP1 protein is one of the subunits and an integral component of Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. It is also involved in smooth muscle contraction. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20 kDa Myosin light chain to MYPT1. PP1c interacts with at least four binding sites on the amino-terminus of MYPT1. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity. Cat. No. ABS1495, Anti-MYPT1 is raised against GST fustion protein containing recombinant chicken MYPT1 protein C-terminal tail fragment (UniProt Q90623) that is present in both isoform 1 (M133, 1004aa) and isoform 2 (M130, 963aa). Human reactivity of this product is confirmed by Western blotting analysis using HeLa cell lysate.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-MYPT1 Antibody is an antibody against MYPT1 for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to chicken MYPT1.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Chicken based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PPP1R12A
  • MBS
  • MYPT1
Purification MethodAffinity
UniProt Number
Molecular Weight~130/140 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:200 dilution of this antibody detected MYPT1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABS1495 04055977173475