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ABE1420 Anti-Mediator complex subunit 25

Anti-Mediator complex subunit 25, Cat. No. ABE1420, is a highly specific rabbit polyclonal antibody that targets MED25 and has been tested in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.

Anti-Mediator complex subunit 25 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: MED25, ACID1, ARC92, PTOV2, TCBAP0758 UniProt Number: Q71SY5
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ABE1420
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIH(P), PIA, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1420
DescriptionAnti-Mediator complex subunit 25
Alternate Names
  • Mediator of RNA polymerase II transcription subunit 25
  • Activator interaction domain-containing protein 1
  • Activator-recruited cofactor 92 kDa component
  • ARC92
Background InformationMediator of RNA polymerase II transcription subunit 25 (UniProt: Q71SY5; also known as Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78) is encoded by the MED25 (also known as ACID1, ARC92, PTOV2, TCBAP0758) gene (Gene ID: 81857) in human. It is a member of the Mediator complex subunit 25 family that is ubiquitously expressed. Higher expression levels are observed in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen. It acts as a coactivator that is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator complex subunit 25 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Six isoforms of Mediator complex subunit 25 have been described that are generated by alternative splicing. Defects in MED25 gene are known to cause Charcot-Marie-Tooth disease 2B2, a disorder of the peripheral nervous system characterized by progressive weakness and atrophy.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Mediator complex subunit 25, Cat. No. ABE1420, is a highly specific rabbit polyclonal antibody that targets MED25 and has been tested in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Peptide Inhibition Assay
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Mediator complex subunit 25 in human cerebellum and mouse cerebral cortex tissues.

Peptide Inhibition Analysis: 2 µg/mL from a representative lot blocked Mediator complex subunit 25 in mouse brain tissue lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the internal region of human Mediator of RNA polymerase II transcription subunit 25 (MED25).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Mediator of RNA polymerase II transcription subunit 25 (MED25) in human and murine species. it targets an epitope within 16 amino acids from the internal region. Detects all isoforms of Mediator complex subunit 25.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Yeast, Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MED25
  • ACID1
  • ARC92
  • PTOV2
  • TCBAP0758
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~70 kda observed; 78.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Mediator complex subunit 25 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE1420 04054839339608