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MAB384-1ML Anti-Myelin Basic Protein Antibody, a.a. 67-74, clone 26

Anti-Myelin Basic Protein Antibody, a.a. 67-74, clone 26 detects level of Myelin Basic Protein & has been published & validated for use in WB, IH.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers, Neurochemistry & Neurotrophins Gene Symbol: MBP, MGC99675, Golli-mbp UniProt Number: P02686
MAB384-1ML
1 mL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMAB384-1ML
ReplacesMAB384
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Myelin Basic Protein Antibody, a.a. 67-74, clone 26
Alternate Names
  • MBP
References
Product Information
FormatCulture Supernatant
PresentationTissue culture supernatant. Liquid containing 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Myelin Basic Protein Antibody, a.a. 67-74, clone 26 detects level of Myelin Basic Protein & has been published & validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern blotting

Immunohistochemistry - on frozen sections.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenHuman myelin basic protein.
Epitopea.a. 67-74
Clone26
HostMouse
SpecificityReacts with an epitope of myelin basic protein between aa 67-74.

Amino acid sequence YGSLPQ. Reacts with MBP from most species.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
Gene Symbol
  • MBP
  • MGC99675
  • Golli-mbp
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.
SIZE: 304 amino acids; 33117 Da
SUBUNIT: Homodimer; isoform 3 exists as a homodimer.
SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.
PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20° in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze-thaw cycles.
Packaging Information
Material Size1 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB384-1ML 04053252613050

Documentation

Anti-Myelin Basic Protein Antibody, a.a. 67-74, clone 26 Certificates of Analysis

TitleLot Number
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) 2465133
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) MONOCLONAL ANTIBODY - 2446675 2446675
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) - 3305140 3305140
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) - 3501834 3501834
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) - 3509173 3509173
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) - 3729450 3729450
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) - 3862676 3862676
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) -2806946 2806946
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) MONOCLONAL ANTIBODY 3008456
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) MONOCLONAL ANTIBODY 2933821
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References

Reference overviewPub Med ID
Region-specific immunoassays for human myelin basic protein.
Groome, N P, et al.
J. Neuroimmunol., 12: 253-64 (1986)  1986

Show Abstract 		2428830 2428830

Data Sheet

Title
MOUSE ANTI-MYELIN BASIC PROTEIN (MBP) (67-74) MONOCLONAL ANTIBODY

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Product Families

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Millipore’s MBP antibodies demonstrate specificity against Myelin basic protein (MBP). See below for related products for MBP, based on the expertise of Upstate & Chemicon.
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Glial Cell Markers

Glial cells, sometimes called neuroglia are non-neuronal cells that maintain homeostasis, form myelin, and provide support and protection for neurons in the brain and peripheral nervous system.
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies