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ABN45 Anti-Myelin Regulatory Factor (MRF) Antibody

This Anti-Myelin Regulatory Factor (MRF) Antibody is validated for use in western blotting & IHC for the detection of Myelin Regulatory Factor (MRF).

Anti-Myelin Regulatory Factor (MRF) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: MYRF, C11orf9, KIAA0954, MRF UniProt Number: Q9Y2G1
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ABN45
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN45
DescriptionAnti-Myelin Regulatory Factor (MRF) Antibody
Alternate Names
  • Myelin regulatory factor
  • Myelin gene regulatory factor
  • Myelin regulatory factor, N-terminal
  • Myelin regulatory factor, C-terminal
Background InformationMyelin Regulatory Factor (MRF), also known as Myelin regulatory factor, MYRF, and encoded by the gene MYRF/C11orf9/KIAA0954/MRF, is an interesting protein that gets cleaved into two chains. The N terminal component specifically activates transcription of myelin gene in the CNS oligodendrocytes and pre-oligodendrocytes. The C-terminal portion, which is remains membrane bound, appears to play no role in gene induction but its complete role is still being discovered. The N-terminal portion of MRF activates MBP, MOG, MAG, and PLP among other genes, but it is not required for oligodendrocyte differentiation but is required for the maintenance of myelin in the adult. MRF whole factor is localized to the endoplasmic reticulum. The cleaved N terminal portion translocates to the nucleus and the C-terminal portion remains in the endoplasmic reticulum. Interestingly MRF can be found expressed outside of oligodendrocytes and oligo precursors in the brain including regions such as the lung, basal ganglion and liver.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Myelin Regulatory Factor (MRF) Antibody is validated for use in western blotting & IHC for the detection of Myelin Regulatory Factor (MRF).
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected Myelin Regulatory Factor (MRF) in 10 µg of ARPE19 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Myelin Regulatory Factor (MRF) in human cerebral cortex, human retina, and human cerebellum tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Myelin Regulatory Factor (MRF).
Epitopecytoplasmic domain, N-terminal chain
Concentration1.0 mg/mL
HostRabbit
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Hamster, Horse, Monkey based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MYRF
  • C11orf9
  • KIAA0954
  • MRF
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~160 kDa observed. The expected molecular weight is 125 kDa and ~160kDa when glycosylated. The protein was cleaved into a ~90 kDa N-terminal fragment from the full length of ~160 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myelin Regulatory Factor (MRF) in 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABN45 04053252977527

Documentation

Anti-Myelin Regulatory Factor (MRF) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Myelin Regulatory Factor (MRF) Antibody Certificates of Analysis

TitleLot Number
Anti-Myelin Regulatory Factor (MRF) - Q2431448 Q2431448
Anti-Myelin Regulatory Factor (MRF) - 3233121 3233121
Anti-Myelin Regulatory Factor (MRF) - 3277599 3277599
Anti-Myelin Regulatory Factor (MRF) - 3389043 3389043
Anti-Myelin Regulatory Factor (MRF) - 3587272 3587272
Anti-Myelin Regulatory Factor (MRF) - 4180375 4180375
Anti-Myelin Regulatory Factor (MRF) -2625370 2625370
Anti-Myelin Regulatory Factor (MRF) -2652011 2652011
Anti-Myelin Regulatory Factor (MRF) -2668508 2668508
Anti-Myelin Regulatory Factor (MRF) -2770274 2770274
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